Human Activin A Precursor MAb (Clone 132815) (25 UG)
货号：
MAB1203-SP 基本售价：
1467.3 元 规格：
-
产品信息
概述| 货号 | MAB1203-SP |
| 别名 | INHBA |
| 反应种属 | Human |
| 应用 | Western Blot |
| 目标/特异性 | Detects human Activin A Precursor in direct ELISAs and Western blots. Recognizes an epitope found in the human Activin A pro region that is removed when the protein is processed to its mature form. |
| 使用方法 | Western Blot: 1 µg/mL
|
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 3624 (Human); 16323 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | DNA construct encoding human Activin A pro region fused to mature human Activin beta B chain |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Activin and Inhibin are members of the TGF-beta superfamily of cytokines and are involved in a wide range of biological processes including tissue morphogenesis and repair, fibrosis, inflammation, neural development, hematopoiesis, reproductive system function, and carcinogenesis (1‑7). Activin and Inhibin are produced as precursor proteins. Their amino terminal propeptides are proteolytically cleaved and facilitate formation of disulfide-linked dimers of the bioactive proteins (8, 9). Activins are nonglycosylated homodimers or heterodimers of various beta subunits ( beta A, beta B, beta C, and beta E in mammals), while Inhibins are heterodimers of a unique alpha subunit and one of the beta subunits. Activin A is a widely expressed homodimer of two beta A chains. The beta A subunit can also heterodimerize with a beta B or beta C subunit to form Activin AB and Activin AC, respectively (10). The 14 kDa mature human beta A chain shares 100% amino acid sequence identity with bovine, feline, mouse, porcine, and rat beta A. Activin A exerts its biological activities by binding to the type 2 serine/threonine kinase Activin RIIA which then noncovalently associates with the type 1 serine/threonine kinase Activin RIB/ALK-4 (7, 11). Signaling through this receptor complex leads to Smad activation and regulation of activin-responsive gene transcription (7, 11). The bioactivity of Activin A is regulated by a variety of mechanisms (11). BAMBI, Betaglycan, and Cripto are cell-associated molecules that function as decoy receptors or limit the ability of Activin A to induce receptor complex assembly (12‑14). The intracellular formation of Activin A can be prevented by the incorporation of the beta A subunit into Activin AC or Inhibin A (3, 10). And the bioavailability of Activin A is restricted by its incorporation into inactive complexes with alpha 2-Macroglobulin, Follistatin, and FLRG (15, 16). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
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