| 货号 | MAB572-SP |
| 别名 | beta-R1; b-R1; chemokine (C-X-C motif) ligand 11; H174; H174IP9; Interferon gamma-inducible protein 9; Interferon-inducible T-cell alpha chemoattractant; IP-9member 11; ITAC; I-TAC; I-TACMGC102770; SCYB9B; small inducible cytokine subfamily B (Cys-X-Cys), member 9B; Small-inducible cytokine B11 |
| 反应种属 | Mouse |
| 应用 | Western Blot,Neutralization |
| 目标/特异性 | Detects mouse CXCL11/I‑TAC in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse CXC1,2, 6, 9, 10, CXCL12/SDF-1 alpha, 13, 14, recombinanthuman CXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, CXCL12/SDF-1 alpha, CXCL12/SDF-1 beta, 13, 14, 15, recombinant rat CXCL1, 2, CXCL3/CINC2 alpha, CXCL3/CINC-2 beta, 5, or recombinant porcine CXCL8 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize CXCL11/I‑TAC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR3. The Neutralization Dose (ND50) is typically 0.4-2.0 µg/mL in the presence of 50 ng/mL Recombinant Mouse CXCL11/I‑TAC. |
| 来源 | Monoclonal Rat IgG2A Clone # 131327 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6373 (Human); 56066 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant mouse CXCL11/I-TAC Phe22-Met100 Accession # Q9JHH5 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | CXCL11 (also known as I-TAC, SCYB9B, H174, IP-9, and beta -R1) is a member within the non-ELR CXC chemokine subgroup and has been designated CXCL11. CXCL11, together with MIG and IP-10, constitute a subset of chemokines that are ligands for CXCR3, a chemokine receptor that is primarily expressed on activated Th1 cells and NK cells. The three chemokines were also reported to act as antagonists for CCR3, a chemokine receptor that is preferentially expressed on activated Th2 cells. Mouse CXCL11 cDNA encodes a 100 amino acid (aa) precursor protein with a putative 21 aa signal peptide that is cleaved to yield a 79 aa mature protein. Mature mouse and human CXCL11 share 71% aa sequence identity. Mouse CXCL11 also shares 36% and 29% aa sequence identity with mouse IP-10 (CRG-2) and mouse MIG, respectively. The gene for mouse CXCL11 has been mapped to chromosome 5, in close proximity to the IP-10 and MIG genes. Mouse CXCL11 is induced in multiple tissues during endoxemia, with the greatest expression in lung, heart, small intestine, and kidney. The endotoxemia-induced mouse CXCL11 expression is strongly attenuated by treatment with glucocorticoid. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Chemotaxis Induced by CXCL11/I‑TAC and Neutralization by Mouse CXCL11/ I‑TAC Antibody. Recombinant Mouse CXCL11/ I‑TAC (Catalog # 572-MC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL11/ I‑TAC (50 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL11/I-TAC Monoclonal Antibody (Catalog # MAB572). The ND50 is typically 0.4‑2.0 µg/mL. |
