| 货号 | MAB573-SP |
| 别名 | IL-1 R6; IL1RL2; IL-1RL2 | 全称 | Interleukin 1 Receptor-like 2 |
| 反应种属 | Rat |
| 应用 | Western Blot |
| 目标/特异性 | Detects rat IL‑1 Rrp2/IL‑1 R6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human (rh) IL-1 Rrp2 is observed and no cross-reactivity with recombinant mouse (rm) IL-1 RI, rmIL-1 RII, rhIL-1 RAcP, rmIL-1 R5, rmIL‑1 R7, and rmIL-1 RAPL1 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8808 (Human); 107527 (Mouse); 171106 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant rat IL‑1 Rrp2/IL‑1 R6 Gly22-Tyr340 Accession # Q62929 |
| 生物活性 | Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL‑1 R3), ST2 (T1/IL-1 R4), IL‑18 Ra (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL-18 Rb (AcPL/IL-1 R7), IL-1RAPL‑1 (TIGIRR‑2/IL‑1 R8), and TIGIRR-1 (IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll‑like receptor family. Related proteins, SIGIRR (single Ig domain-containing IL-1 R-related molecule) and IL-18BP, differ from the other members by having only one Ig domain (1). Rat IL-1 Rrp2 cDNA encodes a 561 amino acid (aa) residue precursor protein with a putative 21 aa signal peptide and a 319 aa extracellular domain. It shares 84% and 67% amino acid sequence identity with mouse and human IL‑1 Rrp2, respectively. IL-1 Rrp2 is expressed in lung epitheium, brain vasculature, kidney, testis, monocytes, skin-derived keratinocytes, fibroblasts and, to a lesser extent, endothelial cells (2, 3). IL‑1 Rrp2 has been shown to mediate the activation of the transcription factor NF‑kB by the IL-1 family ligands IL-1 F6, F8 or F9 (also known as IL-1e), with IL‑1 RAcP as a co-factor (3, 4). Response to IL-1 F9 is specifically antagonized by IL-1 F5 (also known as IL-1d), an IL-1 family ligand that is most closely related to IL‑1ra (3). IL-1 Rrp2, IL-1 F5, and IL-1 F9 are all up-regulated in lesional psoriasis skin, suggesting that the IL-1 Rrp2 mediated signaling pathway may take part in local inflammatory responses (3). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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