| 货号 | MAB369-SP |
| 别名 | EFL4; EFL-4; EFNA4; EPH-related receptor tyrosine kinase ligand 4; ephrin-A4; EPLG4MGC125826; LERK-4; LERK4FLJ57652; ligand of eph-related kinase 4 |
| 反应种属 | Human |
| 应用 | Western Blot,Immunohistochemistry |
| 目标/特异性 | Detects human Ephrin-A4 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross‑reactivity with recombinant mouse (rm) Ephrin-A4 is observed and no cross-reactivity with recombinant human Ephrin-A3, -A5, -B3, rmEphrin-A1, -A2, -B1, and -B2 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1945 (Human); 13639 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Ephrin-A4 Leu26-Gly171 Accession # P52798 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Ephrin-A4, also known as LERK-4 and EFL-4, (1) is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The A-class ligands have a GPI anchor following the conserved sequence. Ephrin-A4 has been shown to bind EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1 (2, 3). The extracellular domains of human and mouse Ephrin-A4 share 80% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Ephrin‑A4 in Human Squamous Cell Carcinoma. Ephrin‑A4 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human Ephrin‑A4 Monoclonal Antibody (Catalog # MAB369) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. |
