| 货号 | MAB3832-SP |
| 别名 | CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein |
| 反应种属 | Human |
| 应用 | Western Blot,Flow Cytometry,CyTOF-ready,Neutralization |
| 目标/特异性 | Detects human CD14 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/106cells Neutralization: Measured by its ability to neutralize lipopolysacharide (LPS)-induced TNF‑ alpha secretion in the THP-1 human acute monocytic leukemia cell line. At 10 µg/mL, this anti-hCD14 antibody will neutralize >60% of 100 ng/mL LPS-induced TNF-alpha secretion. |
| 来源 | Monoclonal Mouse IgG1 Clone # 134620 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Extracellular Vesicle Proteins Associated with Systemic Vascular Events Correlate with Heart Failure: An Observational Study in a Dyspnoea Cohort. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human CD14 Thr20-Cys352 Accession # P08571 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The human CD14 cDNA encodes a 375 amino acid (aa) residue precursor protein with a 19 aa signal peptide and a C-terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Human CD14 has four potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of human CD14 is approximately 65% identical with the mouse, rat, rabbit, and bovine proteins. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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