| 货号 | MAB9439-SP |
| 别名 | Dengue Virus 2/4 NS1 | 全称 | Dengue Virus 2/4 Polyprotein |
| 应用 | Western Blot |
| 目标/特异性 | Detects recombinant Dengue Virus 2 NS1 protein and recombinant Dengue Virus 4 NS1 protein in Western blot and Direct Elisa. Detects recombinant Dengue Virus 1 and 3 NS1 proteins in Direct Elisa. |
| 使用方法 | Western Blot: 5 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1494449 (Viral) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Human embryonic kidney cell line HEK293-derived recombinant Dengue Virus 2 NS1 protein Asp1-Ala352 Accession # NP_739584 |
| 生物活性 | Viral |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Dengue virus (DENV), the cause of Dengue fever, is a single positive-stranded RNA virus of the family Flaviviridae, genus Flavivirus. 5 closely related but antigenically distinct serotypes of the virus have been found: DENV-1, DENV-2, DENV-3, DENV-4 and DENV-5. DENV has a genome of about 11,000 bases, encoding a single large polyprotein that is subsequently cleaved into several structural and non-structural peptides. The polyprotein is divided into three structural proteins, C, prM, E, seven non-structural proteins, NS1, NS2a, NS2b, NS3, NS4a, NS4b, NS5, and short non-coding regions on both the 5 and 3 ends. DENV nonstructural protein-1 (NS1) is a secreted glycoprotein that is absent from viral particles but accumulates in the supernatant and on the plasma membrane of cells during infection. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Dengue Virus 2/4 NS1 by Western Blot. Western blot shows recombinant Dengue Virus (DENV) 1, recombinant DENV2, recombinant DENV3, and recombinant DENV4. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Dengue Virus 2/4 NS1 Monoclonal Antibody (Catalog # MAB9439) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Dengue Virus 2/4 NS1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. |
