Mouse Wnt-9b MAb (Clone 964742) (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Mouse Wnt-9b MAb (Clone 964742) (25 UG)

货号： MAB36691-SP 基本售价： 1457.4 元规格： -

产品信息

概述
货号	MAB36691-SP
别名	Protein Wnt-14b; wingless-type MMTV integration site family, member 15; wingless-type MMTV integration site family, member 9B; Wnt-14b; WNT14Bprotein Wnt-9b; Wnt-15; WNT15Protein Wnt-15; Wnt9b; Wnt-9b
全称	Wingless-type MMTV Integration Site Family, Member 9b
应用	Neutralization
目标/特异性	Detects mouse Wnt-9b in direct ELISAs.
使用方法	Neutralization: Measured by its ability to neutralize Wnt‑9b-induced alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 125-750 ng/mL in the presence of 400 ng/mL Recombinant Mouse Wnt‑9b.
来源	Monoclonal Rat IgG_2B Clone # 964742
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7484 (Human); 22412 (Mouse); 303586 (Rat)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Chinese hamster ovary cell line CHO-derived recombinant mouse Wnt-9b Met1-Arg359 Accession # O35468
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Mouse Wnt-9b, previously called Wnt-14b or Wnt-15, is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved, cysteine-rich secreted glycoproteins important for normal developmental processes (1‑3). Wnts mainly transduce signals by binding to receptors of the Frizzled family, in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6) (1, 2). The 359 aa mouse Wnt-9b precursor contains a 336 aa mature region with 24 conserved cysteines (3). Mature mouse Wnt-9b shares 93%, 98%, 92%, 92% and 91% aa identity with human, rat, canine, equine and bovine Wnt-9b, respectively. It is evolutionarily related to Wnt-9a and Wnt-3, which share 63% and 32% aa identity, respectively (4). Wnt-9b mRNA is expressed in late embryos and in adult kidney, with lesser amounts in brain and liver (3, 4). It appears to direct mesenchymal-to-epithelial transition in the kidney and other tissues (5). During kidney development, it is expressed in the ureteric bud and induces mesonephric and metanephric tubulogenesis, nephron development, and caudal extension of the Mullerian duct, acting upstream of Wnt-4 (5‑7). Induction is dependent on beta -catenin activity, implicating the canonical signaling pathway (7). Mice devoid of Wnt-9b die shortly after birth due to kidney agenesis, while low expression results in small kidneys with fewer nephrons (1, 5, 6). Mutations of Wnt-9b also cause incompletely penetrant cleft lip and palate in mice, indicating its involvement with facial midline morphogenesis (8, 9). It has weak transforming activity compared to other transforming Wnts (4).
运输条件	Blue Ice
存放说明	4℃
参考文献	http://www.stanford.edu/group/nusselab/cgi-bin/wnt/. Logan, C. Y. and R. Nusse (2004) Annu. Rev. Cell Dev. Biol. 20:781. Kirikoshi, H. and M. Katoh (2002) Int. J. Mol. Med. 9:135. Qian, J. et al. (2003) Genomics 81:34. Carroll, T. J. et al. (2005) Dev. Cell 9:283. Merkel, C. E. et al. (2007) Pediatr. Nephrol. 22:1825. Park, J-S. et al. (2007) Development 134:2533. Lan, Y. et al. (2006) Dev. Dyn. 235:1448. Juriloff, D. M. and M. J. Harris (2008) Birth Defects Res. A Clin. Mol. Teratol. 82:63.

参考图片

Alkaline Phosphatase Production Induced by Wnt‑9b and Neutralization by Mouse Wnt‑9b Antibody. Recombinant Mouse Wnt‑9b (Catalog # 3669-WN) induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast in a dose-dependent manner (orange line). Alkaline Phosphatase Production elicited by Recombinant Mouse Wnt‑9b (400 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Wnt‑9b Monoclonal Antibody (Catalog # MAB36691). The ND₅₀ is typically 125-750 ng/mL.