| 货号 | MAB2950-SP |
| 别名 | CD371; CLEC12A; CLL1; CLL-1; C-type lectin domain family 12, member A; DCAL-2; MICL | 全称 | Myeloid Inhibitory C-type Lectin-like Receptor |
| 应用 | Flow Cytometry,CyTOF-ready |
| 目标/特异性 | Detects mouse MICL/CLEC12A in direct ELISAs. |
| 使用方法 | Flow Cytometry: 0.25 µg/106cells CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. |
| 来源 | Monoclonal Rat IgG2A Clone # 812303 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 160364 (Human); 232413 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse MICL/CLEC12A with an N-terminal 9-His tag Thr67-Arg267 Accession # Q504P2 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | MICL, also known as killer cell lectin-like receptor 1 (KLR1), is an inhibitory C-type lectin-like receptor that contains an immunoreceptor tyrosine-based inhibitory motif (ITIM). It is a75 kDa type II membrane protein that is expressed on neutrophils, NK cells, monocytes and dendritic cells. The amino acid sequence of mouse MICL extracellular domain is 53%, 52% and 58% identical to that of human, bovine and canine MICL, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of MICL/CLEC12A in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with either (A) Rat Anti-Mouse MICL/CLEC12A Monoclonal Antibody (Catalog # MAB2950) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Goat Anti-Rat PE-conjugated secondary antibody (Catalog # F0105B) and Rat Anti-Mouse CD11c APC‑conjugated Monoclonal Antibody (Catalog # FAB69501A). Cells were first gated on Rat Anti-Mouse CD8 alpha Alexa Fluor® 488‑conjugated Monoclonal Antibody-positive cells(Catalog # FAB116G, not shown). View our protocol for Staining Membrane-associated Proteins. |
