| 货号 | MAB9535-SP |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | double homeobox 4; double homeobox protein 10; double homeobox protein 4; double homeobox protein 4/10; double homeobox protein DUX10; DUX10; DUX4; DUX4L1 | 全称 | Double Homeobox 4 |
| 应用 | Western Blot,Immunohistochemistry |
| 目标/特异性 | Detects human DUX4 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 3-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 22947 (Human) |
| 纯化方式 | Protein A or G purified from cell culture supernatant |
| 免疫原 | Human DUX4 synthetic peptide |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Human Doublehomeobox 4 (aka DUX4) is a protein encoded by the DUX4 gene. This gene islocated within a D4Z4 repeat array in chromosome 4q35. Each D4Z4 repeat unithas an open reading frame, DUX4, that contains two homeoboxes. Unregulatedexpression of DUX4 in muscle cells is the cause of facioscapulohumeral musculardystrophy (FSHD), a common form of muscular dystrophy in adults that is one ofthe most prevalent genetic diseases of muscle. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human DUX4 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line transfected with human DUX4 untreated (-) or treated (+) with Doxycycline. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human DUX4 Monoclonal Antibody (Catalog # MAB9535) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for DUX4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
DUX4 in Human Testis. DUX4 was detected in immersion fixed paraffin-embedded sections of human testis using Rabbit Anti-Human DUX4 Monoclonal Antibody (Catalog # MAB9535) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. |
