| 货号 | MAB9419-SP |
| 别名 | ADRB3; ADRB3R; adrenergic, beta-3-, receptor; B3AR; beta-3 Adrenergic R; beta-3 adrenergic receptor; beta3 AdrenergicR; beta-3 AdrenergicR; Beta-3 adrenoceptor; Beta-3 adrenoreceptor; BETA3AR; FLJ99960 | 全称 | Beta-3 Adrenergic Receptor |
| 应用 | Flow Cytometry,Immunocytochemistry |
| 目标/特异性 | Stains human beta -3 Adrenergic R/ADRB3 transfectants but not irrelevent transfectants in flow cytometry. |
| 使用方法 | Flow Cytometry: 0.25 µg/106cells Immunocytochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 155 (Human); 11556 (Mouse); 25645 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Human embryonic kidney cell line HEK293-derived transfected with human beta -3 Adrenergic R/ADRB3 Met1-Ser408 Accession # P13945 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | ADRB3 belongs to the G protein-coupled receptor 1 family of beta adrenergic receptors. Beta-adrenergic receptors, including ADRB3, mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. ADRB3 is involved in the regulation of lipolysis and thermogenesis. A variant of ADRB3 seems to be associated with weight gain (obesity). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of beta ‑3 Adrenergic R/ADRB3 in HEK293 Human Cell Line Transfected with Human beta ‑3 Adrenergic R/ADRB3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human beta ‑3 Adrenergic R/ADRB3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human beta ‑3 Adrenergic R/ADRB3 Monoclonal Antibody (Catalog # MAB9419) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins. | |
beta ‑3 Adrenergic R/ADRB3 in Human Adipocytes. beta ‑3 Adrenergic R/ADRB3 was detected in immersion fixed human adipocytes using Mouse Anti-Human beta ‑3 Adrenergic R/ADRB3 Monoclonal Antibody (Catalog # MAB9419) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
