| 货号 | MAB9365-SP |
| 别名 | C7orf15; C7orf15MGC138295; CD112R; MGC104322; MGC138297; MGC2463; poliovirus receptor related immunoglobulin domain containing; Poliovirus receptor-related immunoglobulin domain-containing protein; PVRIG; transmembrane protein PVRIG | 全称 | Poliovirus Receptor Related Immunoglobulin Domain Containing |
| 应用 | Western Blot,Immunocytochemistry |
| 目标/特异性 | Detects human and mouse PVRIG in Western blots. |
| 使用方法 | Western Blot: 2 µg/mL Immunocytochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 79037 (Human); 620147 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Human PVRIG synthtic peptide Accession # Q6DK17 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Human PVRIG (poliovirusreceptor related immunoglobulin domain-containing protein), also known as CD112receptor (CD112R), is an approximately 34 kDa singletransmembrane protein in the poliovirus receptor-likeprotein (PVR) family (1). It is composed of a single extracellularIgV domain, one transmembrane domain, and a long intracellular domain. Theintracellular domain contains two tyrosine residues, one within an ITIM-likemotif that is a potential docking site for phosphatases (1). The extracellular domain sequenceof human and mouse PVRIG have approximately 65% similarity. The human PVRIGgene is preferentially expressed in lymphocytes, such as T cells and NK cells,but not in monocyte derived dendritic cells (1). PVRIG functions as a cellsurface receptor for Nectin-2/CD112, a cell surface protein that is widelyexpressed on antigen-presenting cells and tumor cells. Disrupting thePVRIG/Nectin-2 interaction enhances human T cell response, suggesting PVRIG isa novel checkpoint for human T cells (1). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human and Mouse PVRIG by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, Daudi human Burkitts lymphoma cell line, Raji human Burkitts lymphoma cell line, CEM human T-lymphoblastoid cell line, and TK‑1 mouse T cell lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse PVRIG Monoclonal Antibody (Catalog # MAB9365) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PVRIG at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
PVRIG in MOLT‑4 Human Cell Line. PVRIG was detected in immersion fixed MOLT‑4 human acute lymphoblastic leukemia cell line using Mouse Anti-Human/Mouse PVRIG Monoclonal Antibody (Catalog # MAB9365) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
