| 货号 | MAB91901-SP |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | CCN2; CCN2IGFBP-8; connective tissue growth factor; CTGF; CTGRP; Fisp12; HCS24; Hypertrophic chondrocyte-specific protein 24; IBP-8; IGF-binding protein 8; IGFBP-8; IGFBP8CCN family member 2; Insulin-like growth factor-binding protein 8; MGC102839; NOV2 | 全称 | Connective Tissue Growth Factor |
| 应用 | Western Blot |
| 目标/特异性 | Detects human CTGF/CCN2 in direct ELISAs. Detects human, mouse, and rat CTGF/CCN2 in Western blots. |
| 使用方法 | Western Blot: 2 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1490 (Human); 14219 (Mouse); 64032 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human CTGF/CCN2 Glu247-Ala349 Accession # P29279 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | CTGF belongs to the CCN (CYR61/CTGF/NOV) family of secreted proteinsthat share a common multimodular organization. Each protein containsIGF-binding protein domain, a von Willebrand factor type C domain, athrombospondin type I domain, and a cysteine knot domain. Themultimodular CTGF has the ability to bind multiple ligands and hasnumerous effects on development, differentation, and disease. TheC-terminal cysteine knot motif contains the heparin and low densitylipoprotein receptor (LDLR) binding sites that contributes to theadhesive and mitogenic properties of CTGF. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human, Mouse, and Rat CTGF/CCN2 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, SVEC4‑10 mouse vascular endothelial cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human/Mouse/Rat CTGF/CCN2 Monoclonal Antibody (Catalog # MAB91901) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for CTGF/CCN2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
