| 货号 | MAB57091-SP |
| 别名 | MGC138426; parathyroid hormone 1 receptorMGC138452; parathyroid hormone/parathyroid hormone-related peptide receptor; parathyroid hormone/parathyroid hormone-related protein receptor; PFE; PTH/PTHr receptor; PTH/PTHrP type I receptor; PTH1 receptor; PTH1R; PTHR; PTHR1; PTHRPTHR1parathyroid hormone receptor 1; seven transmembrane helix receptor | 全称 | Parathyroid Hormone Receptor 1 |
| 应用 | Immunohistochemistry |
| 目标/特异性 | Detects human PTHR1 in direct ELISAs. |
| 使用方法 | Immunohistochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5745 (Human); 19228 (Mouse); 56813 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human PTHR1 Tyr23-Met189 Accession # Q03431 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | PTH1R (Parathyroid hormone receptor # 1; also PTH/PTHr receptor) is a predicted 66-68 kDa member of the G-protein coupled receptor family # 2. It is expressed on a variety of cell types, including hepatocytes, renal epithelium, smooth muscle cells, and osteoblasts plus chondrocytes. PTH1R is a receptor for both PTH and PTHrP, and PTH binding promotes Ca++-release from bone, mediated by osteoclast formation. Mature human PTH1R is a 7-transmembrane glycoprotein 567 amino acids (aa) in length. It contains a long, ligand-binding N-terminal extracellular region (aa 27-188) and a 130 aa cytoplasmic C-terminal domain. Single aa changes such as Gly121Glu, Ala122Thr and Arg255His can impair PTH1R signaling. Over aa 1-189, human PTH1R shares 88% aa identity with mouse PTH1R. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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PTH1R/PTHR1 in Human Kidney. PTH1R/PTHR1 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human PTH1R/PTHR1 Monoclonal Antibody (Catalog # MAB57091) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
