| 货号 | MAB5232-SP |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | DER4; Fit-1; IL-1 R4; IL1R4; IL-1R4; IL-1RL1; IL-33R; Ly84; ST2; ST2L; ST2V; suppression of tumorigenicity 2; T1 | 全称 | Interleukin 33 Receptor |
| 应用 | ELISA,Flow Cytometry |
| 目标/特异性 | Detects human ST-2/IL-33R in direct ELISAs. |
| 使用方法 | ELISA: This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human ST2/IL-33 R Monoclonal Antibody (Catalog # MAB5231). This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human ST2/IL-33 R DuoSet ELISA Kit (Catalog # DY523B-05) for convenient development of a sandwich ELISA or the Human ST2/IL-33 R Quantikine ELISA Kit (Catalog # DST200) for a complete optimized ELISA. Flow Cytometry: 0.25 µg/106cells |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9173 (Human); 17082 (Mouse) |
| 纯化方式 | Protein A or G purified from cell culture supernatant |
| 免疫原 | S. frugiperda insect ovarian cell lineSf 21-derived recombinant human ST-2/ IL-33R Lys19-Phe328 Accession # Q01638 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | ST2, also known as IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Human ST2 consists of a 310 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 207 aa cytoplasmic domain with an intracellular TIR domain (3, 4). Alternate splicing of the 120 kDa human ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions as well as an isoform that additionally lacks the third Ig‑like domain (4). Within the ECD, human ST2 shares 68% and 64% aa sequence identity with mouse and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (5‑8). It binds IL-33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (5, 9). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 10, 11). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (7). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (5, 12‑14). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (6, 8, 15). Soluble ST2 functions as a decoy receptor that blocks the ability of IL‑33 to signal through transmembrane ST2 (10, 13‑15). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of ST2/IL-33 R in HEK293 Human Cell Line Transfected with Human ST2/IL-33 R and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human ST2/IL-33 R or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human ST2/IL-33 R Monoclonal Antibody (Catalog # MAB5232) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins. | |
Human ST2/IL-33 R ELISA Standard Curve. Recombinant Human ST2/IL-33 R protein was serially diluted 2-fold and captured by Rabbit Anti-Human ST2/IL-33 R Monoclonal Antibody (Catalog # MAB5232) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human ST2/IL-33 R Monoclonal Antibody (Catalog # MAB5231) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994). |
