Human PKC epsilon MAb (Clone 666843) (25 UG)【科瑞奥博】

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Human PKC epsilon MAb (Clone 666843) (25 UG)

货号： MAB51341-SP 基本售价： 1457.4 元规格： -

产品信息

概述
货号	MAB51341-SP
别名	EC 2.7.11; EC 2.7.11.13; MGC125656; nPKC-epsilon; PKC epsilon; PKCE; PKCEMGC125657; PRKCE; protein kinase C epsilon type; protein kinase C, epsilon
全称	Protein Kinase C epsilon
应用	Western Blot
目标/特异性	Detects human PKC epsilon in direct ELISAs and Western blots.
使用方法	Western Blot: 2 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5581 (Human); 18754 (Mouse); 29340 (Rat)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human PKC epsilon Gln580-Pro737 Accession # Q02156
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	PKC epsilon (protein kinase C-epsilon) is an 87 kDa member of the novel PKC subfamily, AGC Ser/Thr protein kinase family of enzymes. It is a widely-expressed Ca⁺⁺‑insensitive, phospholipid-dependent enzyme that catalyzes the phosphorylation of multiple proteins. Human PKC epsilon is 737 amino acids (aa) in length. It contains two general regions: a non-Ca⁺⁺-binding plus lipid binding regulatory region (aa 1-99 and 169-292, respectively), and an ATP-binding catalytic domain (aa 408-668). Phosphorylation of PKC epsilon on Thr566, Ser368 and Ser729 activates the enzyme and increases its molecular weight to 92 kDa. One potential splice form shows a 114 aa substitution for aa 118-737. Over aa 580-737, human PKC epsilon is 98% and 99% aa identical to mouse and canine PKC epsilon, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Chemokine Receptor Intracellular Signaling Diabetes Intracellular Kinases Intracellular Kinases in the Akt Pathway Intracellular Signaling Molecules in Angiogenesis PDGF Family TNF Superfamily Regulators VEGF Family Wnt Intracellular Signaling

参考图片

Detection of Human PKC epsilon by Western Blot. Western blot shows lysates of Human brain (hippocampus) tissue and human brain (hypothalamus) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PKC epsilon Monoclonal Antibody (Catalog # MAB51341) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PKC epsilon at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.