Human Phospho-STAT3 (Y705) MAb (Clone 1004G) (25 UG)【科瑞奥博】

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Human Phospho-STAT3 (Y705) MAb (Clone 1004G) (25 UG)

货号： MAB46071-SP 基本售价： 1457.4 元规格： -

产品信息

概述
货号	MAB46071-SP
简介	Recombinant Monoclonal Antibody
别名	Acute-phase response factor; APRFMGC16063; DNA-binding protein APRF; FLJ20882; HIES; signal transducer and activator of transcription 3 (acute-phase responsefactor); signal transducer and activator of transcription 3; STAT3
全称	Signal Transducer and Activator of Transcription 3
应用	Western Blot
目标/特异性	Detects human STAT3 when phosphorylated at Y705 in Western blots.
使用方法	Western Blot: 0.1 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6774 (Human); 20848 (Mouse); 25125 (Rat)
纯化方式	Protein A or G purified from cell culture supernatant
免疫原	Phosphopeptide containing the human STAT3 Y705 site Accession # P40763
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Human STAT-3/STAT3a (signal transducer and activator of transcription 3; also called APRF) is a 90-95 kDa member of the STAT family of transcription factors. It is 770 amino acids (aa) in length, contains one SH2 domain (aa 580-670), and is found in almost all cell types. STAT3 mediates gp130, LIF-R, OB-R, IL10-R and EGFR signaling. Upon activation, receptors such as gp130 phosphorylate STAT3 at Ser727, which results in transcriptional activation. Phosphorylation at Tyr705 mediates homo- and hetero-dimerization with STAT1 and nuclear translocation, and is an important event in oncogenic transformation. The STAT3b isoform shows a six aa substitution for aa 716-770, thus eliminating the Ser727 site. Human and mouse STAT3a share 99% aa sequence identity.
运输条件	Blue Ice
存放说明	4℃
参考文献	Apoptosis Transcription Factors and Regulators B Cell Markers Cancer Stem Cell Transcription Factors Chemokine Receptor Intracellular Signaling Common gamma Chain Receptor Family Dendritic Cell Development Embryonic and Induced Pluripotent Stem Cell Markers Epithelial Stem Cell Transcription Factors Focal Adhesions Gametes and Fertilization GDNF Family Hematopoietic Stem Cell Transcription Factors and Regulators IL-10/Interferon Family IL-12 Family Immunoglobulin-Like Domain Transcription Factors Intracellular Signaling Molecules in Angiogenesis Jak and STAT Proteins Mesenchymal Stem Cell Transcription Factors Myeloid-derived Suppressor Cells (MDSC) Neural Stem Cell Transcription Factors PDGF Family Pluripotent Stem Cell Transcription Factors Signaling Molecules and Transcriptional Regulators of Tfh Cell Differentiation STAT Th17 Cells Th22 Cells Transcription Factors in the Akt Pathway Transcriptional Regulators in Allergy and Asthma VEGF Family

参考图片

Detection of Human Phospho-STAT3 (Y705) by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IL‑22 (Catalog #
782-IL) for 15 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-STAT3 (Y705) Monoclonal Antibody (Catalog # MAB46071) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-STAT3 (Y705) at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.