| 货号 | AF1600-SP |
| 别名 | HKAT; Tiki2; TRABD2B TraB domain containing 2B; TRABD2B |
| 应用 | Western Blot,Flow Cytometry |
| 目标/特异性 | Detects human Tiki2/TRABD2B in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL Flow Cytometry: 0.25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 388630 (Human); 689914 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Human embryonic kidney cell line HEK293-derived transfected with human Tiki2/TRABD2B Accession # A6NFA1 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | TIKI2, encoded by the TRABD2B gene, is a 517 aminoacid metalloproteinase that acts as a negative regulator of Wnt signaling by mediating the cleavage of N-terminal residues in many of Wnt proteins. Tiki matalloproteins are dependent on Mn2+/Co2+ and are inhibited by divalent metal chelators like EDTA. The other known Tiki protein in human, Tiki1, is required for head formation via Wnt cleavage-oxidation and inactivation, but lacks known metalloprotease motifs. TIKI2 is upregulated in renal cell carcinoma and it appears to suppress growth of osteosarcoma by targeting the canonical Wnt pathway |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Tiki2/TRABD2B by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human Tiki2/TRABD2B. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Tiki2/TRABD2B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1600) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Tiki2/TRABD2B at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Tiki2/TRABD2B in HEK293 Human Cell Line Transfected with Human Tiki2/TRABD2B by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either human Tiki2/ TRABD2B (filled histogram) or irrelevant transfectants (open histogram) was stained with Sheep Anti-Human Tiki2/TRABD2B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1600), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). View our protocol for Staining Membrane-associated Proteins. |
