| 货号 | AF3120-SP |
| 别名 | HFE3; HFE3transferrin receptor protein 2; MGC126368; TfR2; TFRC2; transferrin receptor 2 | 全称 | Transferrin Receptor 2 |
| 应用 | Western Blot |
| 目标/特异性 | Detects human TfR2 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 2 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7036 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human TfR2 Arg105-Phe801 Accession # Q9UP52 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Transferrin Receptor 2 (TfR2) is an iron-binding protein that is homologous to the transferrin receptor. Lack of active TfR2 has been associated with a rare form of hemochromatosis. The TfR2 alpha isoform is a type 2 transmembrane protein that is expressed in the liver and modulates hepcidin production in response to iron. It forms a 215 kDa disulfide-linked homodimer that is presumably glycated. The TfR2 beta isoform lacks the intracellular, transmembrane and part of the extracellular regions and is expressed ubiquitously. The extracellular portion of human TfR2 shares 86% aa identity with mouse. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human TfR2 by Western Blot. Western blot shows lysates of human liver tissue and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TfR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3120) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TfR2 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
