| 货号 | AF7090-SP |
| 别名 | CARD4; CARD4NLR family, CARD domain containing 1; caspase recruitment domain family, member 4; Caspase recruitment domain-containing protein 4; CLR7.1; NLRC1; NOD1; nucleotide-binding oligomerization domain containing 1; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domaincontaining 1; nucleotide-binding oligomerization domain-containing protein 1 | 全称 | Nucleotide-binding Oligomerization Domain-containing Protein 1 |
| 应用 | Immunohistochemistry |
| 目标/特异性 | Detects human NOD1. |
| 使用方法 | Immunohistochemistry: 1-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10392 (Human); 107607 (Mouse); 500133 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human NOD1 Lys830-Phe953 Accession # Q9Y239 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | NOD1, also called CARD4, is a 108 kDa intracellular pattern recognition receptor of the Nod-like receptor (NLR or Caterpiller) family. This innate immune receptor recognizes the muramyl peptide, diaminopimelic acid. The 953 amino acid (aa) NOD1 contains a caspase recognition domain (CARD), an NTPase domain and 10 leucine‑rich repeats (LRR). Splicing variants of 925 and 779 aa lack all or part of LRR 7-10 and do not respond to muramyl peptides. Polymorphisms of NOD1 are associated with inflammatory bowel disease. Within aa 830-953 (LRR 7-110), human NOD1 shares 82% and 84% aa identity with mouse and rat NOD1, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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NOD1 in Mouse Skeletal Muscle. NOD1 was detected in immersion fixed frozen sections of mouse skeletal muscle using Sheep Anti-Human NOD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7090) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
