| 货号 | AF9055-SP |
| 别名 | D3S1231E; D3S1231ESEC13L1npp-20; Npp-20; protein SEC13 homolog; SEC13 (S. cerevisiae)-like 1; SEC13 homolog (S. cerevisiae); SEC13; SEC13L1; SEC13-like 1 isoform; SEC13-like protein 1; SEC13R; SEC13-related protein; SEC13RSEC13-like 1 (S. cerevisiae) | 全称 | Nuclear Pore Complex Protein Nup85 |
| 应用 | Western Blot,Simple Western,Immunocytochemistry |
| 目标/特异性 | Detects human SEC13 in direct ELISA and Western blots. |
| 使用方法 | Western Blot: 0.05 µg/mL Simple Western: 0.5 µg/mL Immunocytochemistry: 1-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6396 (Human); 110379 (Mouse); 297522 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human SEC13 Gly123-Gln322 Accession # P55735 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Sec13 is a ubiquitously expressed protein which participatesin the formation of vesicles in the COPII complex with Sec23p.Sec24p, Sar1p and Sec31. The COPIIvesicle budding complex was first described in the yeast system and was shownto provide coat proteins in the early secretory pathway. Sec13 is recruited toendoplasmic reticulum (ER) membranes where it forms a three dimensionalcuboctahedron cage lattice structure by association with Sec31. This vesicularstructure is necessary for ER-Golgi transport. Presenillin-1, which isimplicated in early-onset Alzheimers disease has been shown to interact withSec13 vesicles. Additionally, Sec13 has been shown to be a component ofthe Y-shaped Nup107-160 subcomplex of the nuclear pore complex (NPC), and theGATOR2 sub-complex of the GATOR complex. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human SEC13 by Western Blot. Western blot shows lysates of human kidney tissue and human lung tissue. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human SEC13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9055) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SEC13 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
SEC13 in HeLa Human Cell Line. SEC13 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human SEC13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9055) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
Detection of Human SEC13 by Simple WesternTM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for SEC13 at approximately 42 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human SEC13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9055). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
