| 货号 | AF9104-SP |
| 别名 | MGC8946; RING finger protein 15RORETRo/SSA ribonucleoprotein homolog; RNF15; RoRet; TRIM38; tripartite motif containing 38; tripartite motif-containing 38; tripartite motif-containing protein 38; Zinc finger protein RoRet; zinc-finger protein RoRet | 全称 | Tripartite Motif-containing 38 |
| 应用 | Western Blot,Immunohistochemistry |
| 目标/特异性 | Detects human TRIM38 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 2 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10475 (Human); 214158 (Mouse); 680019 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E.coli-derived recombinant human TRIM38 Leu190-Ile414 Accession # O00635 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Human Tripartite Motif 38 (TRIM38, RNF15) is a 465 aminoacids E3 ubiquitin ligase that plays a key role in tuning TLR-mediated immune responses by selectively targeting molecules in the TLR signaling for proteasomal degradation. The TRIM family of E3 ligases has more than 70 members in humans. Many members of this protein family are important immune regulators, mediating innate immune cell signal transduction and subsequent cytokine induction immune regulation. Trim38 deficiency, for example, greatly increases TLR3- and TLR4-mediated induction of type I Interferons and proinflammatory cytokines, such as TNF-alpha, IL-1 beta, and IL-6, in immune cells and in vivo. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human TRIM38 by Western Blot. Western blot shows lysates of RT-4 human bladder carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TRIM38 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9104) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TRIM38 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
TRIM38 in Human Lymph Node. TRIM38 was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human TRIM38 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9104) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. |
