| 货号 | 9664L |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IHC-F/IF-IC/F |
| 目标/特异性 | Cleaved Caspase-3 (Asp175) (5A1) Rabbit mAb detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full length caspase-3 or other cleaved caspases. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:1000) F (1:6400) IF-IC (1:400) IHC-F (1:800) |
| 供应商 | CST |
| 背景 | Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2). |
| 存放说明 | -20C |
| 计算分子量 | 17, 19 |
Immunoprecipitation of extracts from Jurkat cells, untreated or etoposide-treated (25uM, 5hrs), using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb. Western blot was performed using the same antibody. 免疫沉淀分析Jurkat 细胞的提取物，未处理或 etoposide （依托泊苷）(25uM, 5hrs)处理，采用Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗，然后用同样抗体进行western blot 分析。 | |
Immunohistochemical staining of paraffin-embedded mouse embryo, showing cytoplasmic localization in apoptotic cells, using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb. 免疫组化分析石蜡包埋小鼠胚胎，显示凋亡细胞中裂解的caspase-3定位胞浆，抗体采用 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗。 | |
Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with etoposide #2200 (green), using Cleaved Caspase-3(Asp175) (5A1E) Rabbit mAb compared to a nonspecific negative control antibody (red). | |
Immunohistochemical analysis using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb on SignalSlide (TM) Cleaved Caspase-3 IHC Controls #8104 (paraffin-embedded Jurkat cell, untreated (left) or etoposide-treated (right)). 免疫组化分析，用 SignalSlide (TM) Cleaved Caspase-3 IHC Controls #8104（石蜡包埋的Jurkat 细胞，未处理（左）或 etoposide 处理（右）），采用 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗。 | |
Western blot analysis of extracts from C6 (rat), NIH/3T3 (mouse), and Jurkat (human) cells, untreated or treated with staurosporine (1uM, 3hrs) or etoposide (25uM, 5hrs) as indicated, using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb. Western blot 分析未处理或 staurosporine （星胞菌素） (1uM, 3hrs) 或 etoposide （依托泊苷）(25uM, 5hrs)处理的C6 (大鼠源)、NIH/3T3 (小鼠源)和Jurkat (人源) 细胞。抗体采用Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded mouse embryo, using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb in the presence of control peptide (left) or Cleaved Caspase-3 (Asp175) Blocking Peptide (#1050) (right). 免疫组化分析石蜡包埋的小鼠胚胎，抗体采用Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗，并用对照肽段（左图）或 Cleaved Caspase-3 (Asp175) 封闭肽段 (#1050) 对照(右图)。 | |
Confocal immunofluorescent images of HT-29 cells, untreated (left) or Staurosporine treated (right) labeled with Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb#9953 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析未处理（左）或 Staurosporine (星胞菌素)处理（右）的HT-29 细胞，抗体采用 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗#9953（绿色）。微丝用Alexa Fluor® 555 phalloidin（红色）标记。蓝色伪彩=DRAQ5® #4084 (DNA 荧光染料)。 | |
Immunohistochemical analysis of frozen H1650 xenograft, using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb. 免疫组化分析 H1650 xenograft 冰冻切片，抗体采用Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗。 |
