| 货号 | 3633S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 目标/特异性 | ALK (D5F3) XP® Rabbit mAb detects endogenous levels of total ALK protein as well as ALK fusion proteins, such as EML4-ALK variants and NPM-ALK, even at low levels. This antibody does not cross-react with other family members. |
| 使用方法 | WB(1:2000) IP (1:100) IHC-P (1:250) F (1:400) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Anaplastic lymphoma kinase (ALK) is a tyrosine kinase receptor for pleiotrophin (PTN), a growth factor involved in embryonic brain development (1-3). In ALK-expressing cells, PTN induces phosphorylation of both ALK and the downstream effectors IRS-1, Shc, PLCγ, and PI3 kinase (1). ALK was originally discovered as a nucleophosmin (NPM)-ALK fusion protein produced by a translocation (4). Investigators have found that the NPM-ALK fusion protein is a constitutively active, oncogenic tyrosine kinase associated with anaplastic lymphoma (4). Research literature suggests that activation of PLCγ by NPM-ALK may be a crucial step for its mitogenic activity and involved in the pathogenesis of anaplastic lymphomas (5). A distinct ALK oncogenic fusion protein involving ALK and echinoderm microtubule-associated protein like 4 (EML4) has been described in the research literature from a non-small cell lung cancer (NSCLC) cell line, with corresponding fusion transcripts present in some cases of lung adenocarcinoma. The short, amino-terminal region of the microtubule-associated protein EML4 is fused to the kinase domain of ALK (6-8). |
| 存放说明 | -20C |
| 计算分子量 | 220 (ALK), 80 (NPM-ALK), 117 (EML4-ALK v1), 86 (EML4-ALK v3) |
Flow cytometric analysis of DU 145 cells (blue) and KARPAS-299 cells (green) using ALK (D5F3) XP® Rabbit mAb. 流式细胞检测分析DU 145细胞（蓝色）和KARPAS-299细胞（绿色），使用的抗体为ALK (D5F3) XP® Rabbit mAb。 | |
Confocal immunofluorescent analysis of KARPAS-299 cells (left), NCI-H2228 cells (center) and DU 145 cells (right), using ALK (D5F3) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦免疫荧光法检测KARPAS-299细胞（左图），NCI-H2228 细胞(中图)和 DU 145细胞 (右图),使用的抗体为ALK (D5F3) XP® Rabbit mAb (绿色).蓝色伪彩色为DNA荧光染料，产品信息为DRAQ5® #4084. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma with high (left) and low levels (right) of ALK expression using ALK (D5F3) XP® Rabbit mAb. 免疫组织化学方法检测石蜡包埋的人肺癌组织，ALK高水平表达（左图）和低水平表达（右图），使用的抗体为ALK (D5F3) XP® Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded anaplastic large cell lymphoma (ALCL) using ALK (D5F3) XP® Rabbit mAb. 免疫组织化学方法检测石蜡包埋的间变性大细胞淋巴瘤（ALCL），使用的抗体为ALK (D5F3) XP® Rabbit mAb. | |
Western blot analysis of extracts from NCI-H2228 and NCI-H3122 cells using ALK (D5F3) XP® Rabbit mAb. Variants denoting fusions of different EML4 exons (v1 or v3) are indicated. Western blot方法检测NCI-H2228细胞和NCI-H3122细胞提取物，使用的抗体为ALK (D5F3) XP® Rabbit mAb. 表明变种指示不同的EML4外显子（V1或V3）的融合。 |
