| 货号 | 5284L |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/F |
| 目标/特异性 | Phospho-Bad (Ser112) (40A9) Rabbit mAb detects endogenous levels of Bad only when phosphorylated at Ser112. The Ser112 nomenclature is based upon the mouse sequence. The analogous phosphorylation site is Ser75 in human and Ser113 in rat. This antibody does not detect Bad phosphorylated at other sites, nor does it detect related family members. |
| 使用方法 | WB(1:1000) IHC-P (1:50) F (1:800) |
| 供应商 | CST |
| 背景 | Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xl (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation of Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10). |
| 存放说明 | -20C |
| 计算分子量 | 23 |
Immunohistochemical analysis of paraffin embedded COS cells untreated (left) or TPA-treated (right), showing induced cytoplasmic staining using Phospho-Bad (Ser112) (40A9) Rabbit mAb. 免疫组化分析石蜡包埋的未经处理(左图)或者TPA处理 (右图)的COS细胞，图示为诱导的细胞质染色，所用抗体为Phospho-Bad (Ser112) (40A9) Rabbit mAb 兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded Non-Hodgkins lymphoma, using Phospho-Bad (Ser112) (40A9) Rabbit mAb. 免疫组化分析石蜡包埋的非霍奇金淋巴瘤，所用抗体为Phospho-Bad (Ser112) (40A9) Rabbit mAb 兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Phospho-Bad (Ser 112) (40A9) Rabbit Monoclonal Antibody preincubated with control peptide (left) or Phospho-Bad (Ser 112) Blocking Peptide (IHC Specific) #1026 (right). 免疫组化分析石蜡包埋的人前列腺癌组织，经过对照多肽(左图) 或者Phospho-Bad (Ser 112)Blocking Peptide (IHC Specific) #1026 预孵育(右图)后, 再用Phospho-Bad (Ser112) (40A9) Rabbit mAb 兔单抗检测。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, untreated (left) or lambda phosphatase treated (right), using Phospho-Bad (Ser 112) (40A9) Rabbit mAb. 免疫组化分析石蜡包埋的人乳腺癌组织，未经处理(左侧) 或者 lambda 磷酸酶处理(右图), 所用抗体为Phospho-Bad (Ser112) (40A9) Rabbit mAb 兔单抗。 | |
Flow cytometric analysis of COS cells, untreated (blue) or TPA/Calyculin A treated (green), using Phospho-Bad (Ser112) (40A9) Rabbit mAb. 流式细胞仪分析未经处理(蓝色) 或者经过 TPA/Calyculin A 处理(绿色)的COS 细胞的细胞提取液, 所用抗体为Phospho-Bad (Ser112) (40A9) Rabbit mAb 兔单抗。 | |
Western blot analysis of extracts from COS cells, untreated or TPA-treated, using Phospho-Bad (Ser112) (40A9) Rabbit mAb (upper) or Bad Antibody #9292 (lower). Western blot 免疫印迹分析未经处理或经过TPA处理的COS 细胞的细胞提取液，用Phospho-Bad (Ser112) (40A9) Rabbit mAb 兔单抗 (上图) 或者 Bad Antibody #9292 (下图) 。 |
