| 货号 | 9198L |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IHC-F/IF-F/IF-IC/F/ChIP |
| 目标/特异性 | Phospho-CREB (Ser133) (87G3) Rabbit mAb detects endogenous levels of CREB only when phosphorylated at serine 133. The antibody also detects the phosphorylated form of the CREB-related protein, ATF-1. |
| 使用方法 | WB(1:1000) IHC-P (1:800) F (1:800) IF-F (1:800) IF-IC (1:800) IHC-F (1:400) ChIP (1:50) |
| 供应商 | CST |
| 背景 | CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9). |
| 存放说明 | -20C |
| 计算分子量 | 43 |
Flow cytometric analysis of SK-N-MC cells, untreated (blue) or IBMX- and forskolin-treated (green), using Phospho-CREB (Ser133) (87G3) Rabbit mAb compared to a nonspecific negative control antibody (red). 流式细胞仪分析未处理（蓝色）或用IBMX-和forskolin处理（绿色）的SK-N-MC细胞，使用的抗体是Phospho-CREB (Ser133) (87G3) Rabbit mAb 兔单抗和非特异性阴性对照抗体（红色）。 | |
Western blot analysis of extracts from SK-N-MC cells, untreated or forskolin- and FGF-treated, using Phospho-CREB (Ser133) (87G3) Rabbit mAb (upper) or CREB (48H2) Rabbit mAb #9197 (lower). Western blot分析SK-N-MC细胞（未处理或Forskolin和FGF处理），使用的抗体是Phospho-CREB (Ser133) (87G3) Rabbit mAb 兔单抗（上图）或CREB (48H2) Rabbit mAb 兔单抗#9197（下图）。 | |
Confocal microscopic images of SK-N-MC cells showing nuclear stain after 25 minute treatment with Forskolin and IBMX using Phospho-CREB (Ser133) (87G3) Rabbit mAb (left, red) compared to untreated cells (right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对SK-N-MC细胞进行25分钟Forskolin和IBMX处理后，共聚焦显微图像显示出核染色，使用Phospho-CREB (Ser133) (87G3) Rabbit mAb 兔单抗（左图，红色），与未处理对照组比对（右图）。蓝色伪彩=DRAQ5 ® #4084(荧光DNA染料)。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing nuclear staining, using Phospho-CREB (Ser133) (87G3) Rabbit mAb. 免疫组化分析石蜡包埋的人肺癌，使用的抗体是Phospho-CREB (Ser133) (87G3) Rabbit mAb 兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-CREB (Ser133) (87G3) Rabbit mAb in the presence of control peptide (left) or Phospho-CREB (Ser133) Blocking Peptide #1090 (right). | |
免疫组化分析石蜡包埋的人乳腺癌，使用的抗体是Phospho-CREB (Ser133)(87G3) Rabbit mAb 兔单抗，其中左图为存在对照肽，右图为存在Phospho-CREB (Ser133)Blocking Peptide 封闭肽 #1090 。 | |
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma, untreated (left) or lambda phosphatase-treated (right), using Phospho-CREB (Ser133) (87G3) Rabbit mAb. 免疫组化分析石蜡包埋的人肾细胞癌，未处理（左）或λ磷酸酶处理（右），使用的抗体是Phospho-CREB (Ser133) (87G3) Rabbit mAb 兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded SK-N-MC cells, untreated (left) or IBMX- and forskolin-treated (right), showing induced nuclear staining, using Phospho-CREB (Ser133) (87G3) Rabbit mAb. |
