| 货号 | 4407L |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:250) |
| 供应商 | CST |
| 背景 | The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for c-Cbl, an adaptor protein that leads to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provides a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10). |
| 存放说明 | -20C |
| 计算分子量 | 175 |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, untreated (left) or calf-intestinal phosphatase (CIP) treated (right), using Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb.用抗磷酸化EGFR (Tyr1173)的兔源单克隆抗体(53A5)对经过（右）或未经（左）牛小肠磷酸酶(CIP)处理的石蜡包埋的人乳腺癌组织进行免疫组化检测。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb.用抗磷酸化EGFR (Tyr1173)的兔源单克隆抗体(53A5)对石蜡包埋的人乳腺癌组织进行免疫组化检测。 | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Phospho-EGF Receptor (Tyr1173)(53A5) Rabbit mAb (left) or the same antibody preincubated with Phospho-EGF Receptor (Tyr1173) Blocking Peptide (#1175) (right).用抗磷酸化EGFR (Tyr1173)的兔源单克隆抗体(53A5)（左）或同样的抗体与磷酸化EGFR (Tyr1173)的阻断型多肽共孵育之后（右）对石蜡包埋的人前列腺癌组织进行免疫组化检测。 | |
Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb specifically binds to tyrosine phosphorylated EGF receptors but not other phosphorylated tyrosine kinases. Western blot analysis of extracts from cells expressing different activated tyrosine kinase proteins, using Phospho-EGF Receptor (Tyr1173)(53A5) Rabbit mAb (upper) or Phospho-Tyrosine mAb (P-Tyr-100) #9411 (lower).抗磷酸化EGFR (Tyr1173)的兔源单克隆抗体(53A5)能够结合EGFR的酪氨酸磷酸化位点但与其它酪氨酸位点被磷酸化的酪氨酸激酶不发生交叉反应。用抗磷酸化EGFR (Tyr1173)的兔源单克隆抗体(53A5)（上）或抗磷酸化酪氨酸(P-Tyr-100)的单克隆抗体#9411 (下)表达各种不同激活的酪酸激酶的细胞的提取物进行免疫印迹检测。 | |
Immunohistochemical analysis using Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb on SignalSlide™ Phospho-EGF Receptor IHC Controls #8102 (paraffin-embedded KYSE450 cell pellets, untreated (left) or EGF-treated (right)).用抗磷酸化EGFR (Tyr1173)的兔源单克隆抗体(53A5)对磷酸化EGFR免疫组化检测对照切片SignalSlide™#8102进行免疫组化检测：石蜡包埋的经过（右）或未经（左）EGF处理的KYSE450细胞团。 |
