| 货号 | 3597L |
| 反应种属 | Human/Mouse/Rat/Monkey/D.melanogaster |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | Phospho-eIF2alpha (Ser51) RmAb detects endogenous eIF2alpha only when phosphorylated at Ser51. The antibody does not recognize elF2alpha phosphorylated at other sites. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:50) |
| 供应商 | CST |
| 背景 | Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 38 |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb. 使用Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb兔单抗，免疫组化分析人类乳腺癌组织石蜡切片，结果显示磷酸化eIF2alpha蛋白定位在细胞质。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb. 使用Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb兔单抗，免疫组化分析人类肺癌组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded PC-3 cells untreated (left) or thapsigargin-treated (right), using Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb. 使用Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb兔单抗，免疫组化分析PC-3细胞系石蜡切片，细胞分为thapsigargin未处理组（左图）和thapsigargin处理组（右图）。 | |
Western blot analysis of extracts from HeLa and THP-1 cells untreated or phosphatase treated, using Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb. 使用Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb兔单抗，免疫印迹（Western Blot）分析在HeLa和THP-1细胞系中磷酸化eIF2alpha(Ser51)的蛋白水平，细胞分为未处理组和磷酸酶处理组。. | |
Western blot analysis of immunoprecipitates from PC12 cells, using Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb. Lane 1 is lysate control, lane 2 is the antibody alone as negative control and lane 3 is antibody immunocomplex of PC12 cells. 使用Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb兔单抗，免疫印迹（Western Blot）分析在PC12细胞系中免疫沉淀反应。第1道是裂解物参照，第2道是单独的抗体作为阴性对照，第3道PC12细胞的抗体免疫复合物。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb in the presence of control peptide (left) or Phospho-eIF2alpha (Ser51) Blocking Peptide (#1221) (right). 使用Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb兔单抗，免疫组化分析人类肺癌组织石蜡切片，其分别孵育对照肽(左图)和Phospho-eIF2alpha (Ser51) Blocking Peptide (#1221) (右图)。 | |
Western blot analysis of extracts from HT29 (human) and AR42J (mouse) cells, untreated or thapsigargin-treated (300 nM, 30 min), using Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb (upper) or eIF2alpha Antibody #9722 (lower). 使用Phospho-eIF2alpha (Ser51) (119A11) Rabbit mAb兔单抗 (上图)和eIF2alpha Antibody #9722 (下图)，免疫印迹（Western Blot）分析在HT29 (人)和AR42J (小鼠)细胞系中磷酸化eIF2alpha和eIF2alpha蛋白水平，细胞分为thapsigargin未处理组和thapsigargin处理组(300 nM, 30分钟)。 |
