| 货号 | 9327L |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-GSK-3alpha/beta (Ser21/9) (37F11) Rabbit mAb (GSK-3α Preferred) preferentially detects endogenous levels of GSK-3α when phosphorylated at Ser21 and also detects GSK-3β when phosphorylated at Ser9. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). |
| 存放说明 | -20C |
| 计算分子量 | 51, 46 |
Western blot analysis of extracts from COS-7 cells, lamda phosphatase- or PDGF-treated, using Phospho-GSK-3α/β (Ser21/9) (37F11) Rabbit mAb (GSK-3alpha Preferred) (upper) or GSK-3alpha Antibody #9338 (lower). Western blot 分析 COS-7 细胞， λ磷酸酶和PDGF处理组，所用抗体为Phospho-GSK-3a/b (Ser21/9) (37F11) Rabbit mAb兔单抗 (GSK-3a Preferred) (上) 或 GSK-3a Antibody #9338 (下)。 |
