| 货号 | 4791L |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | Phospho-HER3/ErbB3 (Tyr1289) (21D3) Rabbit mAb detects endogenous HER3/ErbB3 proteins only when phosphorylated at tyrosine 1289. The antibody does not cross-react with other phosphorylated receptor tyrosine kinases. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:1000) |
| 供应商 | CST |
| 背景 | HER3/ErbB3 is a member of the ErbB receptor protein tyrosine kinase family, but lacks tyrosine kinase activity. Tyrosine phosphorylation of ErbB3 depends on its association with other ErbB tyrosine kinases. Upon ligand binding, heterodimers form between ErbB3 and other ErbB proteins and ErbB3 is phosphorylated on tyrosine residues by the activated ErbB kinase (1,2). There are at least 9 potential tyrosine phosphorylation sites in the carboxy-terminal tail of ErbB3. These sites serve as consensus binding sites for signal transducing proteins, including Src family members, Grb2, and the p85 subunit of PI3 kinase, which mediate ErbB downstream signaling (3). Both Tyr1222 and Tyr1289 of ErbB3 reside within a YXXM motif and participate in signaling to PI3 kinase (4). ErbB3 is highly expressed in many cancer cells (5) and activation of the ErbB3/PI3K pathway is correlated with malignant phenotypes of adenocarcinomas (6). In tumor development, ErbB3 may function as an oncogenic unit together with other ErbB members (e.g. ErbB2 requires ErbB3 to drive breast tumor cell proliferation) (7). Thus, inhibiting interaction between ErbB3 and ErbB tyrosine kinases has become a novel strategy for anti-tumor therapy. |
| 存放说明 | -20C |
| 计算分子量 | 185 |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-HER3/ErbB3 (Tyr1289)(21D3) Rabbit mAb.免疫组化法检测石蜡包埋的人乳腺癌组织，使用抗体为：Phospho-HER3/ErbB3 (Tyr1289)(21D3) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-HER3/ErbB3 (Tyr1289) (21D3) Rabbit mAb.免疫组化法检测石蜡包埋的人肺腺癌组织，使用抗体为：Phospho-HER3/ErbB3 (Tyr1289)(21D3) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, untreated (left) or Calf Intestinal Phosphatase (CIP)-treated (right), using Phospho-HER3/ErbB3 (Tyr1289)(21D3) Rabbit mAb.免疫组化法检测石蜡包埋的人前列腺癌组织：左，未处理；右，Calf Intestinal Phosphatase (CIP)-处理过的。使用抗体为：Phospho-HER3/ErbB3 (Tyr1289)(21D3) Rabbit mAb。 | |
Phospho-HER3/ErbB3 (Tyr1289) (21D3) Rabbit mAb specifically binds to phosphorylated HER3/ErbB3 but not other phosphorylated tyrosine kinases. Western blot analysis of extracts from cells expressing different activated tyrosine kinase proteins, using Phospho-HER3/ErbB3 (Tyr1289) (21D3) Rabbit mAb (upper) or Phospho-Tyrosine mAb (P-Tyr-100) #9411 (lower).抗体Phospho-HER3/ErbB3 (Tyr1289) (21D3) Rabbit mAb特异结合磷酸化的HER3/ErbB3但不与其它磷酸化酪氨酸激酶结合。Western blot法检测表达不同激活酪氨酸激酶蛋白的细胞的提取物，使用抗体为：Phospho-HER3/ErbB3 (Tyr1289) (21D3) Rabbit mAb（上）或Phospho-Tyrosine mAb (P-Tyr-100) #9411（下）。 | |
Immunohistochemical analysis of paraffin-embedded MDA-MB-453 cells, untreated (left) or neuregulin-treated (right), using Phospho-HER3/ErbB3 (Tyr1289)(21D3) Rabbit mAb.免疫组化法检测石蜡包埋的MDA-MB-453细胞：左，未处理的；右，neuregulin处理的。使用抗体为：Phospho-HER3/ErbB3 (Tyr1289)(21D3) Rabbit mAb。 | |
Western blot analysis of extracts from mIMCD-3 cells, untreated or treated with neuregulin-1 (100 ng/ml, 5 min) using Phospho-HER3/ErbB3 (Tyr1289) (21D3) RmAb. |
