| 货号 | 4631L |
| 反应种属 | Human/Mouse/Rat/Monkey/D.melanogaster |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC |
| 使用方法 | WB(1:1000) IHC-P (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10). |
| 存放说明 | -20C |
| 计算分子量 | 43 |
Western blot analysis of extracts from Jurkat, NIH/3T3 and PC12 cells, untreated or treated as indicated, using Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb..Western blot检测细胞提取物：未经处理和按要求处理的Jurkat, NIH/3T3，PC12细胞，是用的抗体是Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb。 | |
Immunohistochemistry of paraffin-embedded lung carcinoma, showing nuclear localization, using Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb.免疫组织化学方法检测石蜡包埋的人肺癌组织，显示了细胞核的位置，使用的抗体为Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb。 | |
Immunohistochemistry of paraffin-embedded colon carcinoma, showing nuclear localization, using Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb.免疫组织化学方法检测石蜡包埋的人结肠癌组织，显示了细胞核的位置，使用的抗体为Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb。 | |
Immunohistochemistry of paraffin-embedded breast carcinoma, using Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb (left) or the same antibody preincubated with antigen phospho-peptide (right).免疫组织化学方法检测石蜡包埋的人乳腺癌组织。左侧使用的抗体为Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb，右侧使用的是提前用抗原磷酸化肽段孵育的相同抗体。 | |
Immunohistochemistry of paraffin-embedded human glioblastoma, untreated (left) or CIP phosphatase-treated (right), using Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb.免疫组织化学方法检测石蜡包埋的人恶性胶质瘤组织，左侧是未经处理的样品，右侧是CIP磷酸酶处理的样品。使用的抗体为Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb。 | |
Immunohistochemistry of paraffin-embedded NIH/3T3 cells, untreated (left) or anisomycin-treated (right), using Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb.免疫组织化学方法检测石蜡包埋的NIH/3T3细胞。左侧是未经处理的样品，右侧是茴香霉素处理的样品。使用的抗体为Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb。 | |
Confocal immunofluorescent analysis of HeLa cells -/+ UV light, labeled with Phospho-p38 MAPK (green). Absence of staining in untreated cells (left) and nuclear localization in treated cells (right). Red = Actin filaments (phalloidin).p38 MAP激酶 (MAPK), 也被称为 RK (1)、CSBP (2)，是哺乳动物体内酵母HOG激酶的同源物，它参与信号传递级联反应，该级联反应能控制细胞对细胞因子和应激反应的应答(1-4)。p38 MAPK的四种异构体, p38α, β, γ (也被称为Erk6、SAPK3), 和δ (也被称为SAPK4)已经被鉴定出来。与SAPK/JNK途径相似，p38 MAPK由各种细胞应激反应激活，包括渗透压休克 、炎性反应、细胞因子、LPS、UV照射和生长因子。(1-5) MKK3, MKK6,SEK磷酸化p38 MAPK蛋白的苏氨酸（180位点）和酪氨酸（182位点）并使其活化。活化的p38 MAPK能将MAPKAP激酶磷酸化、活化 (3)，并磷酸化转录因子ATF-2(5), Max (6), and MEF2 (5-8)。SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole)是p38 MAPK的选择性抑制子。该复合物能通过p38 MAPK抑制MAPKAPK-2的活化和HSP27后续磷酸化 (9)。SB203580通过结合到ATP接合点上而抑制p38 MAPK的摧毁活性，但是不能通过上游激酶而抑制p38 MAPK的磷酸化(10)。 |
