| 货号 | 9167L |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F/ChIP |
| 目标/特异性 | Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb detects endogenous levels of Stat1 only when phosphorylated at tyrosine 701. The antibody detects phosphorylated tyrosine 701 of p91 Stat1 and also the p84 splice variant. It does not cross-react with the corresponding phospho-tyrosines of other Stat proteins. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:800) F (1:200) IF-IC (1:400) ChIP (1:100) |
| 供应商 | CST |
| 背景 | The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation. |
| 存放说明 | -20C |
| 计算分子量 | 84, 91 |
Western blot analysis of extracts from HeLa cells untreated or treated with interferon-α (IFN-α), using Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (upper) or Stat1 Antibody (#9172) (lower).Western免疫印迹。用Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (上图) 或 Stat1 Antibody (#9172) (下图). 研究未经处理的和经IFN-α处理的 HeLa 细胞的细胞提取液。 | |
Immunohistochemical analysis of paraffin-embedded Non-Hodgkins lymphoma control (left) or λ phosphatase treated (right), using Phospho-Stat1 (tyr701) (58D6) Rabbit mAb.免疫组织化学染色分析石蜡包埋人非霍奇金淋巴瘤，未经处理(左图) 或经λ磷酸酶处理 (右图), 所用抗体为Phospho-Stat1 (tyr701) (58D6) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded stomach (chronic gastritis), using Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb.免疫组织化学染色分析石蜡包埋人慢性胃炎,所用抗体为Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb。 | |
Flow cytometric analysis of HeLa cells, untreated (blue) or IFN-α treated (green), using Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb compared to a nonspecific negative control antibody (red).流式细胞仪研究未经处理的HeLa细胞(蓝色)和经IFN-alpha处理的HeLa细胞(绿色)。所用抗体为Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb和非特异性阴性对照抗体(红色)。 | |
Immunohistochemical analysis of paraffin-embedded Non-Hodgkin’s lymphoma using Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167 in the presence of control peptide (left) or Phospho-Stat1 (Tyr701) Blocking Peptide (right).免疫组织化学染色分析石蜡包埋人非霍奇金淋巴瘤。在对照多肽(左图) 或Phospho-Stat1 (Tyr701)封闭多肽的存在下用抗体Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167 进行研究。 | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or IFNα-treated #9906 (right), using Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光。用Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (绿色)研究未经处理(左图) 或者经IFNα 处理(右图)的HeLa细胞。蓝色= DRAQ5™ ( DNA 荧光染料)。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HT-1080 cells treated with IFN-γ (50 ng/ml) for 30 minutes and either 5 μl of Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP® Human TAP1 Promoter Primers #5148, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫共沉淀。HT-1080 细胞培养至4 x 106 , 并经IFN-γ (50 ng/ml,30 min) 处理,然后用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003进行免疫沉淀实验,本实验中用5 μl of Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb 抗体或2 μl Normal Rabbit IgG #2729 抗体。用IRF-1 promoter primers, SimpleChIP® Human TAP1 Promoter Primers #5148, 和 SimpleChIP® Human α Satellite Repeat Primers #4486对富集的DNA做real-time PCR。每个样本中沉淀的DNA量定义为相对信号与输入的总染色质相比的数值。 | |
Immunohistochemical analysis using Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb on SignalSlide® Phospho-Stat1/3/5 IHC Controls #8105 (paraffin-embedded HeLa cell pellets, untreated (left), treated with Human Interferon-α1 (hIFN-α1) #8927 (middle), or treated with Human Epidermal Growth Factor (hEGF) #8916 (right). |
