| 货号 | 2478L |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC |
| 目标/特异性 | Phospho-VEGF Receptor-2 (Tyr1175) (19A10) Rabbit mAb detects endogenous levels of VEGFR-2 proteins only when phosphorylated at tyrosine 1175. This antibody may cross-react with VEGFR1. |
| 使用方法 | WB(1:1000) IHC-P (1:300) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, Grb2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The phosphorylation of Tyr1212 provides a docking site for Grb2 binding and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8). |
| 存放说明 | -20C |
| 计算分子量 | 230 |
Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb specifically binds to phosphorylated VEGFR2, but not other phosphorylated tyrosine kinases. Western blot analysis of extracts from cells expressing different activated tyrosine kinase proteins, using Phospho-VEGF Receptor-2 (Tyr1175) (19A10) Rabbit mAb (upper) and Phospho-Tyrosine mAb (P-Tyr-100) #9411 (lower). CKR/PAE cells (lanes 12 and 13) express chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGF receptor-2 intracellular domain (7). CSF-1 stimulates phosphorylation of Tyr1175 of intracellular VEGF receptor-2 domain (lane 12), which was specifically detected by Phospho-VEGF Receptor-2 (Tyr1175) (19A10) Rabbit mAb.Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb 特异结合磷酸化的VEGFR2, 但不与其它磷酸化的酪氨酸激酶反应。用Phospho-VEGF Receptor-2 (Tyr1175) (19A10) Rabbit mAb (上)和 Phospho-Tyrosine mAb (P-Tyr-100) #9411 (下)对表达不同的活化的酪氨酸激酶蛋白的细胞提取物进行免疫印迹检测。CKR/PAE 细胞 (第12 和13道) 表达含有人的CSF-1胞外结合区/小鼠VEGF receptor 2胞内区的嵌合受体(7)。Phospho-VEGF Receptor-2 (Tyr1175) (19A10) Rabbit mAb 能特意地检测CSF-1刺激下VEGF receptor-2胞内区的Tyr1175 发生磷酸化 (第12道)。 | |
Immunohistochemical analysis of paraffin-embedded HUVE cells, untreated (left) or VEGF treated (right).对未经处理的（左）或经过VEGF处理的（右）石蜡包埋的HUVE细胞进行免疫组化检测。 | |
Western blot analysis of extracts from HUVE cells, untreated or stimulated with VEGF (50 ng/ml for 2 minutes), using Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb (upper) and VEGF Receptor 2 (55B11) Rabbit mAb #2479 (lower).用Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb (上)和 VEGF Receptor 2 (55B11) Rabbit mAb #2479 (下)对未经处理的或经过VEGF (50 ng/ml 处理2 minutes)的HUVE细胞的提取物进行免疫印迹检测。 |
