Cleaved PARP (Asp214) (19F4) Mouse mAb (Human Specific)
货号：
9546L 基本售价：
9300.0 元 规格：
-
产品信息
概述| 货号 | 9546L |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | W |
| 目标/特异性 | Cleaved PARP (Asp214) (19F4) Mouse mAb (Human Specific) detects endogenous levels of the large fragment (89 kDa) of human PARP1 resulting from cleavage at aspartic acid 214. This antibody will detect high levels of full length PARP. |
| 使用方法 | WB(1:2000) |
性能| 供应商 | CST |
| 背景 | PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6). |
| 存放说明 | -20C |
| 计算分子量 | 89 |
参考图片Western blot analysis of extracts from Jurkat cells, untreated or etoposide-treated (25 µM, 5hrs), using Cleaved PARP (Asp214) (19F4) Mouse mAb (Human Specific). 采用Cleaved PARP (Asp214) (19F4) Mouse mAb (Human Specific)鼠单抗对未处理或依托泊苷(25 µM, 5hrs)处理的Jurkat细胞进行免疫印迹分析（western blot）。 |
