| 货号 | 2855L |
| 反应种属 | Human/Mouse/Rat/Monkey/D.melanogaster |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F |
| 目标/特异性 | Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. |
| 使用方法 | WB(1:1000) IHC-P (1:1600) F (1:50) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5). |
| 存放说明 | -20C |
| 计算分子量 | 15 to 20 |
Immunohistochemical analysis of paraffin-embedded human lymphoma using Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb. 使用Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb兔单抗，免疫组化分析人类淋巴瘤组织石蜡切片。 | |
Immunohistochemical analysis using Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb on SignalSlide (TM) Phospho-Akt (Ser473) IHC Controls #8101 (paraffin-embedded LNCaP cells untreated (left) or LY294002-treated (right)). 使用Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb兔单抗，免疫组化分析SignalSlide (TM) Phospho-Akt (Ser473) IHC Controls #8101 (未处理(左图) 和LY294002处理 (右图)的石蜡包埋的LNCaP细胞。 | |
Flow cytometric analysis of Jurkat cells, untreated (green) or LY294002, Wortmannin and U0126-treated (blue), using Phospho-4E-BP1 (Thr36/46) (236B4) Rabbit mAb compared to a nonspecific negative control antibody (red). 与阴性非特异的对照抗体（红色）比较，使用Phospho-4E-BP1 (Thr36/46) (236B4) Rabbit mAb兔单抗，流式细胞术分析Jurkat细胞，细胞分为未处理组（绿色）或LY294002、Wortmannin和U0126处理组（蓝色）。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb. 使用Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb兔单抗，免疫组化分析人类结肠癌组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb in the presence of control peptide (left) or Phospho-4E-BP1 (Thr37/46) Blocking Peptide #1052 (right). 使用Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb兔单抗，免疫组化分析人类结肠癌组织石蜡切片，其分别孵育对照多肽(左图)和Phospho-4E-BP1 (Thr37/46) Blocking Peptide #1052 (右图)。 | |
Confocal immunofluorescent analysis of HeLa cells treated with LY294002 (left) or 20% serum (right) and labeled with Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). 使用Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb兔单抗（绿色），共聚焦免疫荧光观察Miwi蛋白在HeLa细胞中定位，细胞分别使用LY294002处理(左图)或20%血清处理(右图)。Alexa Fluor® 555 phalloidin标记微丝蛋白（红色）。 | |
Western blot analysis of extracts from 293T cells using 4E-BP1 Antibody #9452 (upper) and Phospho-4E-BP1 (Thr37/46) Antibody #2855 (lower). The cells were starved for 24 hours in serum-free medium and underwent a 1 hour amino acid deprivation. Amino acids were replenished for 1 hour. Cells were then either untreated (-) or treated with 100 nM insulin (+) for 30 minutes. 使用4E-BP1 Antibody #9452 (上图)和Phospho-4E-BP1 (Thr37/46) Antibody #2855 (下图)，免疫印迹（Western Blot）分析293 T细胞系裂解物。细胞在无血清饥饿培养24小时，接着氨基酸缺乏培养1小时，然后再加入氨基酸培养1小时。最后，细胞加入100 nM胰岛素 (+)或不加(-)处理30分钟。 |
