| 货号 | 9614L |
| 反应种属 | Human/Mouse/D.melanogaster/All |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/E-P |
| 目标/特异性 | Phospho-(Ser/Thr) Akt Substrate Motif (RXXS*/T*)(110B7) RmAb recognizes peptides and proteins containing phospho-serine/threonine preceded by arginine at the -3 position. There is some preference observed for peptides that contain phospho-serine/threonine preceded by arginine at both positions -5 and -3. (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:1000) E-P (1:1000) |
| 供应商 | CST |
| 背景 | An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation (3-5). While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery. Akt phosphorylates substrates only at Ser/Thr in a conserved motif characterized by Arg at positions -5 and -3 (6). Phospho-Akt substrate-specific antibodies from CST are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery. |
| 存放说明 | -20C |
Western blot analysis of extracts from serum-starved NIH/3T3 cells, phosphorylated in vitro with Akt kinase, or treated in culture with PDGF or FBS/Calyculin A, using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb. 对血清饥饿处理的NIH/3T3细胞抽提液，使用Akt在体外磷酸化，或PDGF或FBS/Calyculin A培养，使用Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb进行Western blot分析。 | |
Immunoprecipitation of extracts from serum-starved NIH/3T3 cells, untreated or treated with FBS/Calyculin A, using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb, followed by Western blot analysis using the same antibody. 对血清饥饿的NIH/3T3细胞抽提液，未处理或使用FBS/Calyculin A处理，使用Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb进行免疫共沉淀，然后用相同的抗体进行Western blot分析后。 | |
Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (T* and S* denote phosphorylated threonine and serine.) Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb ELISA分析：磷酸化和非磷酸化的信噪比。（T*和S*代表磷酸化的苏氨酸和丝氨酸） | |
Immunohistochemical analysis of paraffin-embedded lung carcinoma, using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb. 对石蜡包埋的人肺癌使用Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded renal cell carcinoma, using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb. 对石蜡包埋的人肾癌使用Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma control (left) or lambda phosphatase-treated (right), using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb. 对石蜡包埋的人乳腺癌对照（左）和λ磷酸酶处理（右），使用Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb进行免疫组化分析。 |
