| 货号 | 3313L |
| 反应种属 | Human/Mouse/Rat/Monkey/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | Phospho-Cofilin (Ser3) (77G2) Rabbit mAb detects endogenous levels of cofilin only when phosphorylated at serine 3. |
| 使用方法 | WB(1:1000) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Cofilin and ADF (actin-depolymerization factor) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response, and tissue regeneration (1). In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at Ser3 of cofilin (3-5). Phosphorylation at Ser3 also regulates cofilin translocation from the nucleus to the cytoplasm (6). |
| 存放说明 | -20C |
| 计算分子量 | 19 |
Confocal immunofluorescent analysis of MCF7 cells either untreated (left) or λ phosphatase-treated (right), using Phospho-Cofilin (Ser3) (77G2) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用Phospho-Cofilin (Ser3) (77G2) Rabbit mAb (绿色)标记，共聚焦免疫荧光分析MCF7细胞，细胞分为untreated组 (左图)和λ phosphatase-treated组(右图)。蓝色= DRAQ5® #4084（DNA荧光染料）。 | |
Western blot analysis of NIH/3T3 cells, λ phosphatase-treated or untreated, and various other cell lines, using Phospho-Cofilin (Ser3) (77G2) Rabbit mAb.使用Phospho-Cofilin (Ser3) (77G2) Rabbit mAb，免疫印迹（Western Blot）分析NIH/3T3细胞系中Phospho-Cofilin (Ser3)蛋白水平。 | |
Western blot analysis of NIH/3T3 cells, λ phosphatase-treated or untreated, and various other cell lines, using Phospho-Cofilin (Ser3) (77G2) Rabbit mAb.使用Phospho-Cofilin (Ser3) (77G2) Rabbit mAb，免疫印迹（Western Blot）分析NIH/3T3细胞系中Phospho-Cofilin (Ser3)蛋白水平。 |
