Phospho-EGF Receptor (Tyr1068) (1H12) Mouse mAb【科瑞奥博】

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Phospho-EGF Receptor (Tyr1068) (1H12) Mouse mAb

货号： 2236L 基本售价： 9300.0 元规格： -

产品信息

概述
货号	2236L
反应种属	Human/Rat
来源宿主	Mouse
应用	W/IP/IHC-P
使用方法	WB(1:1000) IP (1:100) IHC-P (1:6400)

性能
供应商	CST
背景	The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for c-Cbl, an adaptor protein that leads to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provides a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10).
存放说明	-20C
计算分子量	175

参考图片

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing membrane localization, using Phospho-EGF Receptor (Tyr1068) (1H12) Mouse mAb.用抗磷酸化EGF受体(Tyr1068) 的小鼠源单克隆抗体 (1H12 ) 对石蜡包埋的人乳腺肿瘤组织进行免疫印组化检测，可见胞膜着色。

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, untreated (left) or calf intestinal phosphatase (CIP) treated (right), using Phospho-EGF Receptor (Tyr1068) (1H12 ) Mouse mAb.用抗磷酸化EGF受体(Tyr1068) 的小鼠源单克隆抗体 (1H12 ) 对石蜡包埋的未经（右）或经过牛小肠磷酸酶处理的（左）人乳腺肿瘤组织进行免疫印组化检测。

Immunohistochemical analyis of paraffin-embedded human glioma using Phospho-EGF Receptor (Tyr1068) (1H12) Mouse mAb.用抗磷酸化EGF受体(Tyr1068) 的小鼠源单克隆抗体 (1H12 ) 对石蜡包埋的神经胶质瘤进行免疫印组化检测。

Western blot analysis of extracts from A431 cells, untreated or EGF-stimulated (100 ng/ml), using Phospho-EGF Receptor (Tyr1068) (1H12) Mouse mAb (upper) or EGF Receptor (C74B9) Rabbit mAb #2646 (lower).用抗磷酸化EGF受体(Tyr1068) 的小鼠源单克隆抗体 (1H12 ) （上）或抗EGFR的兔源单克隆抗体#2646 (C74B9) （下）对下列细胞提取物进行免疫印迹检测：经过或未经EGF (100 ng/ml) 处理的A431 细胞的裂解液。

Immunohistochemical analysis using Phospho-EGF Receptor (Tyr1068) (1H12) Mouse mAb on SignalSlide™ Phospho-EGF Receptor IHC Controls #8102 (paraffin-embedded KYSE450 cell pellets, untreated (left) or EGF-treated (right). 用抗磷酸化EGF受体(Tyr1068) 的小鼠源单克隆抗体(1H12)对SignalSlide™ Phospho-EGF Receptor IHC Controls #8102 即石蜡包埋的经过（右）或未经（左）EGF处理的KYSE450细胞团块进行免疫组化检测。