| 货号 | 9570L |
| 反应种属 | Human/Bovine/Pig |
| 来源宿主 | Rabbit |
| 应用 | W/IP/E-P |
| 目标/特异性 | Phospho-eNOS (Ser1177) (C9C3) Rabbit mAb detects endogenous levels of eNOS only when phosphorylated at Ser1177. |
| 使用方法 | WB(1:1000) IP (1:25) E-P (1:1000) |
| 供应商 | CST |
| 背景 | Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and H2O2 activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 140 |
Western blot analysis of extracts from BAEC cells, phosphatase or VEGF-treated, using Phospho-eNOS (Ser1177) (C9C3) Rabbit mAb (upper) or eNOS Antibody #9572 (lower). Western blot分析BAEC 细胞的提取物物，磷酸酶处理组和VEGF处理组，所用抗体为Phospho-eNOS (Ser1177) (C9C3) Rabbit mAb 兔单抗(上) 或者 eNOS antibody (下)。 |
