Phospho-FGF Receptor (Tyr653/654) (55H2) Mouse mAb
货号：
3476L 基本售价：
9300.0 元 规格：
-
产品信息
概述| 货号 | 3476L |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | W |
| 目标/特异性 | Phospho-FGF Receptor (Tyr653/654) (55H2) Mouse mAb detects transfected levels of FGF receptors only when phosphorylated at tyrosines 653/654. The antibody cross-reacts slightly with activated PDGF and insulin/IGF-I receptors. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3, and FGFR-4. Each receptor contains an extracellular ligand binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR-1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCγ (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 120, 145 |
参考图片Western blot analysis of extracts from COS cells overexpressing human FGF receptor-1, untreated or calf intestine phosphatase (CIP)-treated, using Phospho-FGF Receptor (Tyr653/654) (55H2) Mouse mAb. Overexpression of human FGF receptor-1 results in constitutive activation of the receptors (courtesy of Dr. Pamela Maher, Scripps Research Institute, California, personal communication). CIP treatment abolishes the reactivity of this antibody to FGF receptor-1.Western blot法检测高表达人FGFR1的COS细胞提取物：经过或未经calf intestinal phosphatase (CIP)处理。使用抗体为：Phospho-FGF Receptor (Tyr653/654) (55H2) Mouse mAb 。细胞高表达人FGFR-1蛋白后使受体组成型激活(courtesy of Dr. Pamela Maher, Scripps Research Institute, California, personal communication)。CIP 处理则能破坏本抗体对FGFR-1的反应。 |
Western blot analysis of extracts from A-204 cells treated with or without bFGF, using Phospho-FGF Receptor (Tyr653/654) (55H2) Mouse mAb (upper) and FGF Receptor 1 (D8E4) XP® Rabbit mAb (lower). |
