Phospho-IκBα (Ser32/36) (5A5) Mouse mAb
货号：
9246L 基本售价：
9300.0 元 规格：
-
产品信息
概述| 货号 | 9246L |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Mouse |
| 应用 | W |
| 目标/特异性 | Phospho-IκBα (Ser32/36) (5A5) Mouse mAb detects endogenous levels of IκBα only when phosphorylated at Ser32/36. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8). |
| 存放说明 | -20C |
| 计算分子量 | 40 |
参考图片Western blot analysis of extracts from NIH/3T3 cells, untreated orTNF-α-treated (#2169, 20 ng/ml) for 5 minutes, using Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (upper) or IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) #4814 (lower).Western免疫印迹。用Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (上图) 或者 IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) #4814 (下图)研究未经处理或经TNF-α处理 (#2169, 20 ng/ml，5 min) 的NIH/3T3 细胞的细胞提取液。 |
Western blot analysis of extracts from THP-1 cells, differentiated with TPA (#9905, 80 nM for 24 h) and treated with 1 μg/ml LPS for the indicated times, using Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (upper) and IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) #4814 (lower).Western免疫印迹。用Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (上图) 和 IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) #4814 (下图).研究经TPA (#9905, 80 nM for 24 h)分化和 1 μg/ml LPS 处理一定时间的THP-1细胞的细胞提取液。 |
