| 货号 | 9624L |
| 反应种属 | All |
| 来源宿主 | Rabbit |
| 应用 | W/IP/E-P |
| 目标/特异性 | Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb detects peptides and proteins containing a phospho-Ser/Thr residue with arginine at the -3 and -2 positions. It is a useful tool in identifying new substrates of PKA. The antibody recognizes other -3 arginine-bearing phospho-Ser/Thr peptides, such as substrate motifs for Akt and PKC, to a lesser extent. It does not recognize the nonphosphorylated substrate motif peptides. (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| 使用方法 | WB(1:1000) IP (1:50) E-P (1:1000) |
| 供应商 | CST |
| 背景 | An important class of kinases, refered to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Phospho-PKA substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by PKA and other Arg-directed kinases, as well as for high throughput kinase drug discovery. |
| 存放说明 | -20C |
Western blot analysis of extracts from serum-starved A431 cells, phosphorylated in vitro with PKA kinase or treated in culture with forskolin/IBMX, using Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb. Lysis buffer: 1.0% Triton X-100 (lanes 1 and 2), 2.0% SDS (lanes 3 and 4). 对血清饥饿处理的A431细胞抽提液，使用PKA在体外磷酸化，或forskolin/IBMX培养，使用Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb进行Western blot分析。裂解缓冲液：1.0% Triton X-100 (列1和列2), 2.0% SDS (列3和列4)。 | |
Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (T* and S* denote phosphorylated threonine and serine.) Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb ELISA分析：磷酸化和非磷酸化的信噪比。（T*和S*代表磷酸化的苏氨酸和丝氨酸） |
