EGF Receptor (D38B1) XP® Rabbit mAb【科瑞奥博】

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EGF Receptor (D38B1) XP® Rabbit mAb

货号： 4267L 基本售价： 8800.0 元规格： -

产品信息

概述
货号	4267L
反应种属	Human/Mouse/Monkey
来源宿主	Rabbit
应用	W/IP/IHC-P/IF-IC/F
目标/特异性	EGF Receptor (D38B1) XP® Rabbit mAb detects endogenous levels of total EGF receptor protein. The antibody does not cross-react with other proteins of the ErbB family.
使用方法	WB(1:1000) IP (1:100) IHC-P (1:50) F (1:50) IF-IC (1:50)

性能
供应商	CST
背景	The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for c-Cbl, an adaptor protein that leads to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provides a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10).
存放说明	-20C
计算分子量	175

参考图片

Western blot analysis of extracts from A-431, BxPC3 and HeLa cells using EGF Receptor (D38B1) XP® Rabbit mAb.用抗EGFR的兔源单克隆抗体(D38B1) XP®对A-431, BxPC3 和 HeLa 细胞的提取物进行免疫印迹检测。

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using EGF Receptor (D38B1) XP® Rabbit mAb.用抗EGFR的兔源单克隆抗体(D38B1) XP®对石蜡包埋的人肝癌组织进行免疫组化检测。

Immunohistochemical analysis of paraffin-embedded human placenta using EGF Receptor (D38B1) XP® Rabbit mAb.用抗EGFR的兔源单克隆抗体(D38B1) XP®对石蜡包埋的人胎盘组织进行免疫组化检测。

Immunohistochemical analysis of paraffin-embedded MDA-MB-468 (amplified EGFR, left), HT-29 (low EGFR, middle) and CAMA-1 (EGFR negative, right) cells using EGF Receptor (D38B1) XP® Rabbit mAb.用抗EGFR的兔源单克隆抗体(D38B1) XP®对石蜡包埋的MDA-MB-468 (高表达EGFR, 左), HT-29 (低表达EGFR, 中) 和 CAMA-1 (EGFR阴性, 右)的细胞进行免疫组化检测。

Confocal immunofluorescent analysis of A549 cells, untreated (left) or treated with human epidermal growth factor (right), using EGF Receptor (D38B1) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).用抗EGFR的兔源单克隆抗体(D38B1) XP®（绿色）对经过（右）或未经（左）EGF处理的A549 细胞进行免疫共聚焦检测。蓝色 = DRAQ5® #4084进行的DNA染色。

Flow cytometric analysis of Jurkat cells (red) and Kyse70 cells (blue), using EGF Receptor (D38B1) XP® Rabbit mAb.用抗EGFR的兔源单克隆抗体(D38B1) XP®对Jurkat 细胞 (红色) and Kyse70细胞（蓝色）进行流式细胞术分析。

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using EGF Receptor (D38B1) XP® Rabbit mAb.用抗EGFR的兔源单克隆抗体(D38B1) XP®对石蜡包埋的人肺腺癌组织进行免疫组化检测。