Jak2 (D2E12) XP® Rabbit mAb【科瑞奥博】

产品中心

当前位置：首页>产品中心

Jak2 (D2E12) XP® Rabbit mAb

货号： 3230L 基本售价： 8800.0 元规格： -

产品信息

概述
货号	3230L
反应种属	Human/Mouse/Rat
来源宿主	Rabbit
应用	W/IP/IHC-P/IF-IC/F
目标/特异性	Jak2 (D2E12) XP® Rabbit mAb detects endogenous levels of total Jak2 protein. No cross-reactivity was observed with other family members.
使用方法	WB(1:1000) IP (1:100) IHC-P (1:400) F (1:100) IF-IC (1:100)

性能
供应商	CST
背景	Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3 and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4) and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.
存放说明	-20C
计算分子量	125

参考图片

Flow cytometric analysis of K-562 cells using Jak2 (D2E12) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).流式细胞仪研究K-562细胞，所用抗体为Jak2 (D2E12) XP® Rabbit mAb (蓝色) 与非特异性的对照抗体(红色)。

Confocal immunofluorescent analysis of K-562 cells using Jak2 (D2E12) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).共聚焦免疫荧光分析K-562 细胞，所用抗体为 Jak2 (D2E12) XP® Rabbit mAb (绿色)。 Blue pseudocolor = DRAQ5® (DNA 荧光染料)。

Western blot analysis of extracts from K-562, THP-1, TF-1 and BaF3 cell lines using Jak2 (D2E12) XP® Rabbit mAb.Western免疫印迹。用Jak2 (D2E12) XP® Rabbit mAb研究K-562, THP-1, TF-1 和 BaF3 细胞系的细胞提取液。

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Jak2 (D2E12) XP® Rabbit mAb.免疫组织化学染色分析石蜡包埋人肺癌组织。所用抗体为Jak2 (D2E12) XP® Rabbit mA 。

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Jak2 (D2E12) XP® Rabbit mA in the presence of control peptide (left) or Jak2 Blocking Peptide #1039 (right).免疫组织化学染色分析石蜡包埋人肺癌组织。在对照多肽(左图) 或 Jak2 封闭多肽(右图)存在下所用抗体为Jak2 (D2E12) XP® Rabbit mA 。

Western blot analysis of extracts from K-562 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or Jak2 siRNA (+), using Jak2 (D2E12) XP® Rabbit mAb #3230 and α-Tubulin (11H10) Rabbit mAb #2125. The Jak2 (D2E12) XP® Rabbit mAb confirms silencing of Jak2 expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Jak2 siRNA.Western免疫印迹。用Jak2 (D2E12) XP® Rabbit mAb #3230 和 α-Tubulin (11H10) Rabbit mAb #2125研究转染了100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) 或Jak2 siRNA (+)的K-562细胞的细胞提取液。Jak2 (D2E12) XP® Rabbit mAb 证实对Jak2 表达的沉默, α-Tubulin (11H10) Rabbit mAb作为上样量和Jak2 siRNA特异性的对照。