| 货号 | 2479L |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-F/IF-IC |
| 目标/特异性 | VEGF Receptor 2 (55B11) Rabbit Monoclonal Antibody detects endogenous levels of VEGF receptor 2 protein. This antibody does not cross-react with other family members. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:600) IF-F (1:200) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, Grb2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The phosphorylation of Tyr1212 provides a docking site for Grb2 binding and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8). |
| 存放说明 | -20C |
| 计算分子量 | 210, 230 |
Immunohistochemical analysis of paraffin-embedded breast angiosarcoma, using VEGF Receptor 2 (55B11) Rabbit mAb (left). A serial section is stained for CD31 (PECAM-1), an endothelial cell marker (right). 用VEGF Receptor 2 (55B11) Rabbit mAb (左)对石蜡包埋的乳腺恶性血管皮内细胞瘤进行免疫组化检测。右图显示一系列对内皮细胞分子标记CD31 (PECAM-1)进行染色后的切片。 | |
Western blot analysis of extracts from various cell lines and primary cell cultures, using VEGF Receptor 2 (55B11) Rabbit mAb. PAE/CKR cells overexpress chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGF receptor 2 intracellular domains (Rahimi, N. et al. [2000] J. Biol. Chem. 275, 16986-16992). PAE/VEGFR1 cells overexpress human VEGF receptor 1.用VEGF Receptor 2 (55B11) Rabbit mAb对各种细胞系和原代细胞提取物进行免疫印迹检测。PAE/CKR 细胞过表达含人CSF-1胞外区/小鼠VEGF receptor 2胞内区的嵌合受体 (Rahimi, N. et al. [2000] J. Biol. Chem. 275, 16986-16992). PAE/VEGFR1细胞过表达人的VEGF receptor 1。 | |
Immunohistochemical analysis of paraffin-embedded human renal adenocarcinoma, using VEGF Receptor 2 (55B11) Rabbit mAb. 用VEGF Receptor 2 (55B11) Rabbit mAb对石蜡包埋的人肾脏腺癌进行免疫组化检测。 | |
Immunohistochemical analysis of paraffin-embedded human astrocytoma, using VEGF Receptor 2 (55B11) Rabbit mAb. 用VEGF Receptor 2 (55B11) Rabbit mAb 对石蜡包埋的人星形细胞瘤进行免疫组化检测。 | |
Immunohistochemical analysis of paraffin-embedded HUVEC cells, using VEGF Receptor 2 (55B11) Rabbit mAb.用VEGF Receptor 2 (55B11) Rabbit mAb 对石蜡包埋的HUVEC进行免疫组化检测。 | |
Immunohistochemical analysis of paraffin-embedded HT-29 xenograft, using VEGF Receptor 2 (55B11) Rabbit mAb. Note staining of mouse blood vessels.用VEGF Receptor 2 (55B11) Rabbit mAb 对石蜡包埋的HT-29异种移植物进行免疫组化检测。 |
