Akt1 (2H10) Mouse mAb
货号：
2967L 基本售价：
8070.0 元 规格：
-
产品信息
概述| 货号 | 2967L |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Mouse |
| 应用 | W/IP |
| 目标/特异性 | The Akt1 isoform specific (2H10) Mouse mAb detects endogenous levels of Akt1, regardless of its phosphorylation state and does not cross-react with Akt 2 and Akt 3. |
| 使用方法 | WB(1:1000)
IP (1:100) |
性能| 供应商 | CST |
| 背景 | Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19).Three mammalian Akt isoforms have been identified: Akt1, Akt2, and Akt3. Akt2 and Akt3 have 81% and 83% identity in amino acid sequences with Akt1, respectively (18). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
参考图片Western blot analysis of Akt1 (lane1), Akt2 (lane2) and Akt3 (lane3) recombinant proteins, and extracts of NIH/3T3, HeLa, and COS cells, using Akt1 (2H10) Mouse mAb.western blot方法检测Akt1 (第一泳道), Akt2 (第二泳道) and Akt3 (第三泳道)重组蛋白和 NIH/3T3, HeLa, 和COS细胞提取物；使用的抗体为Akt1 (2H10) Mouse mAb。 |
