| 货号 | 3287S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 目标/特异性 | ROS1 (D4D6) Rabbit mAb recognizes endogenous levels of total ROS1 protein. This antibody does not cross-react with other related proteins when analyzed by western blot. Please note that staining may be observed in ROS1 rearranged lung carcinomas, macrophages/giant cells, reactive type II pneumocyte hyperplasia, and the epithelium in areas of bronchiolar metaplasia. Staining of unknown specificity has been observed in cholangiocarcinoma, hepatocellular carcinoma, and kidney tissues. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:250) F (1:100) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | ROS1, an orphan receptor tyrosine kinase of the insulin receptor family, was initially identified as a homolog of v-ros from the UR2 sarcoma virus (1). ROS1 consists of a large extracellular domain that is composed of six fibronectin repeats, a transmembrane domain, and an intracellular kinase domain. While the function of ROS1 is undefined, it has been shown to play an important role in differentiation of epididymal epithelium (2). The first oncogenic fusion of ROS1, FIG-ROS1, was initially identified by research studies in glioblastoma (3), and subsequent studies have found this fusion in cholangiocarcinoma (4), ovarian cancer (5) and non-small cell lung cancer (NSCLC) (6). Investigators have found additional oncogenic ROS1 fusion proteins in NSCLC (at a frequency of ~1.6%), where the ROS1 kinase domain is fused to the amino-terminal region of a number of different proteins, including CD74 and SLC34A2 (6-8). ROS1 fusion proteins activate the SHP-2 phosphatase, PI3K/Akt/mTOR, Erk, and Stat3 pathways (3,4,9). |
| 存放说明 | -20C |
| 计算分子量 | 258, 110, 50-80 |
Western blot analysis of extracts from HCC78 (SLC34A2-ROS1), U-118 MG (FIG-ROS1), and HeLa (ROS1 negative) cells using ROS1 (D4D6) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Note: HCC78 cells express the 85, 70, and 59 kDa forms of the SLC34A2-ROS1 fusion protein (7).Western blot方法检测HCC78 细胞(SLC34A2-ROS1), U-118 MG细胞 (FIG-ROS1)和 HeLa细胞(ROS1阴性)提取物，使用的抗体为ROS1 (D4D6) Rabbit mAb (上图)或β-Actin (D6A8) Rabbit mAb #8457 (下图)。注意：HCC78细胞表达85, 70和59 kDa的SLC34A2-ROS1融合蛋白压型(7)。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using ROS1 (D4D6) Rabbit mAb. Note: Staining is of FIG-ROS1 fusion (6).免疫组织化学方法检测石蜡包埋的人肺癌组织，使用的抗体为ROS1 (D4D6) Rabbit mAb。注意：染色的是FIG-ROS1融合蛋白(6)。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinomas showing distinct localization using ROS1 (D4D6) Rabbit mAb.免疫组织化学方法检测石蜡包埋的人肺癌组织，显示出明显的位置，使用的抗体为ROS1 (D4D6) Rabbit mAb。 | |
Flow cytometric analysis of HeLa (blue) and HCC78 (green) cells using ROS1 (D4D6) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.流式细胞术方法检测HeLa细胞(蓝色)和HCC78细胞(绿色)，使用的抗体为ROS1 (D4D6) Rabbit mAb.使用的二抗为Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412。 | |
Immunohistochemical analysis of paraffin-embedded HCC78 xenograft using ROS1 (D4D6) Rabbit mAb.免疫组织化学方法检测石蜡包埋的HCC78细胞移植物，使用的抗体为ROS1 (D4D6) Rabbit mAb. | |
Confocal immunofluorescent analysis of HCC78 (left) or HeLa (right) cells using ROS1 (D4D6) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光方法检测HCC78细胞(左图)或HeLa细胞(右图)，使用的抗体为ROS1 (D4D6) Rabbit mAb (绿色)。肌动蛋白丝用DY-554鬼笔环肽标记（红色）。蓝色伪彩色为DNA荧光染料，产品信息为DRAQ5® #4084。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing wildtype ROS1 (hROS1; +), using ROS1 (D4D6) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot方法检测293T细胞提取物，细胞不转染（-）或用表达野生型ROS1(hROS1; +)的构建体进行转染，使用的抗体为ROS1 (D4D6) Rabbit mAb(上图)或β-Actin (D6A8) Rabbit mAb #8457 (下图)。 | |
Immunoprecipitation of ROS1 from HCC78 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or ROS1 (D4D6) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using ROS1 (D4D6) Rabbit mAb, followed by Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 and Anti-mouse IgG, HRP-linked Antibody #7076.免疫沉淀方法检测HCC78细胞提取物中的ROS1，使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2)或ROS1 (D4D6) Rabbit mAb (泳道3)。泳道1是加入10%蛋白。进行Western blot分析使用的抗体为ROS1 (D4D6) Rabbit mAb，随后用Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678和Anti-mouse IgG, HRP-linked Antibody #7076. |
