EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb【科瑞奥博】

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EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb

货号： 3197S 基本售价： 4926.0 元规格： -

产品信息

概述
货号	3197S
反应种属	Human
来源宿主	Rabbit
应用	W/IP/IHC-P/IF-P/IF-IC/F
目标/特异性	EGFR (L858R Mutant Specific) (43B2) Rabbit mAb detects endogenous levels of EGFR mutant L858R protein. Careful titration of this antibody may be required to obtain optimal specificity.
使用方法	WB(1:1000) IP (1:200) IHC-P (1:100) F (1:100) IF-IC (1:400) IF-P (1:400)

性能
供应商	CST
背景	The epidermal growth factor (EGF) receptor is a 170 kDa transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Somatic mutations in the tyrosine kinase domain of EGF receptor (EGFR) are present in a subset of lung adenocarinomas that respond to EGFR inhibitors such as gefinitib and erlotinib (1-3). Two types of mutations account for approximately 90% of mutated cases: a specific point mutation, L858R, that occurs in exon 21 and short in-frame deletions in exon 19 (4,5). The most frequent exon 19 deletion is E746-A750, accounting for 90% of lesions at this site, although some rare variants occur.
存放说明	-20C
计算分子量	175

参考图片

Confocal immunofluorescent analysis of H3255 (left), HCC827 (middle) and A431 cells (right) using EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb (green) and MEK1/2 (L38C12) Mouse mAb #4694 (red). H3255 cells have the L858R EGFR mutation, HCC827 cells have an exon 19 EGFR deletion, and A431 cells overexpress wild type EGFR. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).用抗EGFR（L858R特异突变）的兔源单克隆抗体(43B2)（绿色）和抗MEK1/2的小鼠源单克隆抗体#4694（红色）对H3255 （左）, HCC827（中）和A431（右）细胞进行免疫共聚焦检测。H3255 细胞有L858R EGFR突变，HCC827 细胞有EGFR1的19外显子缺失，而 A431细胞高表达野生型的EGFR。蓝色 = DRAQ5® #4084进行的DNA染色

Western blot analysis of extracts from various EGFR expressing cell lines, showing the specificity of detection of mutant EGFR (L858R) protein using EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb (upper), and total EGFR control antibody (lower).对各种表达EGFR的细胞样品进行免疫印迹检测，可以看到用抗EGFR（L858R特异突变）的兔源单克隆抗体(43B2) （上）能特异性地检测到突变的EGFR (L858R)蛋白，抗总EGFR的对照抗体的检测结果（下）。

Immunohistochemical analysis of paraffin-embedded H1975 (left, EGFR L858R positive) and H1650 xenograft (right, EGFR deletion positive) using EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb (upper) and total EGFR (D38B1) XP® Rabbit mAb #4267 (lower).用抗EGFR（L858R特异突变）的兔源单克隆抗体(43B2)（上）和抗EGFR的兔源单克隆抗体(D38B1) XP® #4267（下）对石蜡包埋的H1975 (左, EGFR L858R 突变) 和 H1650 (右, EGFR 缺失突变) 细胞的异种移植物进行免疫组化检测。

Immunohistochemical analysis of paraffin-embedded human lung carcinoma of known mutational status using EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb (upper) and total EGFR (D38B1) XP® Rabbit mAb #4267 (lower). EGFR L858R positive lung (left), EGFR deletion positive lung sections (right).用抗EGFR（L858R特异突变）的兔源单克隆抗体(43B2)（上）和抗EGFR的兔源单克隆抗体(D38B1) XP® #4267（下）对石蜡包埋的已知突变位点的人肺腺癌组织进行免疫组化检测。EGFR L858R 突变的肺 (左), EGFR缺失突变的肺（右）组织切片。

Confocal immunofluorescent analysis of paraffin-embedded H3255, H1975, HCC827 and H1650 xenografts using EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb (upper, green) and EGFR (D38B1) XP® Rabbit mAb #4267 (lower, green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).用抗EGFR（L858R特异突变）的兔源单克隆抗体(43B2)（上，绿色）和抗EGFR的兔源单克隆抗体(D38B1) XP® #4267（下，绿色）对石蜡包埋的H3255， H1975, HCC827 和 H1650 细胞的异种移植物进行免疫共聚焦检测。蓝色 = DRAQ5® #4084进行的DNA染色。