| 货号 | 2085S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-P/IF-IC/F |
| 目标/特异性 | EGF Receptor (E746-A750del Specific) (6B6) XP® Rabbit mAb detects endogenous levels of EGFR E746-A750del mutant protein. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:100) F (1:1000) IF-IC (1:250) IF-P (1:250) |
| 供应商 | CST |
| 背景 | Epidermal growth factor receptor (EGFR) is a 170 kDa transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Somatic mutations in the tyrosine kinase domain of EGFR are present in a subset of lung adenocarcinomas (1-3). Two types of mutations account for approximately 90% of mutated cases: a specific point mutation L858R which occurs in exon 21 and short in-frame deletions in exon 19 (4,5). A common lesion in exon 19 is the deletion of E746-A750, although other variants occur. |
| 存放说明 | -20C |
| 计算分子量 | 175 |
Confocal immunofluorescent analysis of HCC827, H1650 and Kyse450 cells using EGF Receptor (E746-A750del Specific) (D6B6) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).用抗EGFR（E746-A750特异缺失）的兔源单克隆抗体XP®(6B6) （绿色）对石蜡包埋的HCC827, H1650和Kyse450 细胞进行免疫共聚焦检测。蓝色 = DRAQ5® #4084进行的DNA染色 | |
Western blot analysis of extracts from various EGFR expressing cell lines, showing the specificity of detection of mutant EGFR (E746-A750del) protein, using EGF Receptor (E746-A750del Specific) (6B6) XP® Rabbit mAb (upper), and total EGFR control antibody (lower).如图所示用抗EGFR（E746-A750特异缺失）的兔源单克隆抗体XP®(6B6) （上）和抗总的EGFR的抗体（下）对各种表达EGFR细胞系的裂解液中突变的EGFR（E746-A750特异缺失）的蛋白进行特异性的免疫印迹检测。 | |
Flow cytometric analysis of Kyse450 cells (wildtype, blue) and HCC827 cells (exon 19 deletion (E746-A750del, green) using EGF Receptor (E746-A750del Specific) (6B6) XP® Rabbit mAb.用抗EGFR（E746-A750特异缺失）的兔源单克隆抗体XP®(6B6) 对Kyse450 细胞 (野生型，蓝色) 和 HCC827 cells (19外显子E746-A750缺失，绿色)进行流式细胞术检测。 | |
Immunohistochemical analysis of paraffin-embedded H1975 (left, EGFR L858R positive) and H1650 xenografts (right, EGFR deletion positive) using EGF Receptor (E746-A750del Specific) (6B6) XP® Rabbit mAb (upper) and total EGFR (D38B1) XP® Rabbit mAb #4267 (lower).用抗EGFR（E746-A750特异缺失）的兔源单克隆抗体XP®(6B6) （上）和抗总的EGFR的抗体XP®#4267 (D38B1)（下）对石蜡包埋的H1975 (左， EGFR L858R 阳性) 和 H1650 (右， EGFR缺失) 的异种移植物进行免疫组化检测。 | |
Immunohistochemical analysis of paraffin embedded human lung carcinoma of known mutational status using EGF Receptor (E746-A750del Specific) (6B6) XP® Rabbit mAb (upper) and total EGFR (D38B1) XP® Rabbit mAb #4267 (lower). EGFR L858R positive lung (left), EGFR deletion positive lung sections (right).用抗EGFR（E746-A750特异缺失）的兔源单克隆抗体XP®(6B6) （上）和抗总的EGFR的抗体XP®#4267 (D38B1)（下）对石蜡包埋的已知突变情况的人肺腺癌组织进行免疫组化检测：EGFR L858R 阳性的肺(左) 和EGFR缺失阳性的肺(右)。 | |
Confocal immunofluorescent analysis of paraffin-embedded H3255, H1975, HCC827 and H1650 xenografts using EGF Receptor (E746-A750del Specific) (6B6) XP® Rabbit mAb (upper, green) and EGFR (D38B1) XP® Rabbit mAb #4267 (lower, green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).用抗EGFR（E746-A750特异缺失）的兔源单克隆抗体XP®(6B6) （上，绿色）和抗总的EGFR的抗体XP®#4267 (D38B1)（下，绿色）对石蜡包埋的H3255, H1975, HCC827和 H1650 的异种移植物进行免疫共聚焦检测。蓝色 = DRAQ5® #4084进行的DNA染色 |
