| 货号 | 35652S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | H M R Mk Z |
| 来源宿主 | Rabbit IgG |
| 应用 | IF-IC F |
| 目标/特异性 | Acetyl-α-Tubulin (Lys40) (D20G3) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of α-tubulin only when acetylated at Lys40. This amino acid is not conserved in β-tubulin. |
| 使用方法 | F(1:50) IF-IC (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). |
| 运输条件 | 0.75 |
| 存放说明 | 4C |
| 参考文献 | 1 . Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47. |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Trichostatin A #9950 (right), using Acetyl-α-Tubulin (Lys40) (D20G3) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Flow cytometric analysis of HeLa cells, untreated (blue) or treated with Trichostatin A (TSA) (400 nM, overnight) using Acetyl-α-Tubulin (Lys40) (D20G3) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate). |
