| 货号 | 70034S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse, Rat, Monkey, Zebrafish, Dog |
| 应用 | IF,F |
| 目标/特异性 | Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of β-catenin protein only when Ser45 is not phosphorylated. This direct conjugate failed to meet in-house performance critera for immunofluorescence on fixed-frozen tissue (IF-F). For this purpose, we suggest indirect detection using Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb #19807. |
| 使用方法 | Immunofluorescence (Immunocytochemistry) (1:50) Flow Cytometry (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8). |
| 存放说明 | 4C |
| 参考文献 | 1 . Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305. 2 . Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88. 3 . Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21. 4 . Amit, S. et al. (2002) Genes Dev 16, 1066-76. 5 . Liu, C. et al. (2002) Cell 108, 837-47. 6 . Yanagawa, S. et al. (2002) EMBO J 21, 1733-42. 7 . Yost, C. et al. (1996) Genes Dev 10, 1443-54. 8 . Morin, P.J. et al. (1997) Science 275, 1787-90. |
Confocal immunofluorescent analysis of SW480 (left and lower right; positive) or NCI-H28 (upper right; negative) cells using Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) in the presence of phospho-β-Catenin (Ser45) peptide (lower left) or non-phospho-β-Catenin peptide (lower right). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Flow cytometric analysis of NCI-H28 cells (blue) and SW480 cells (green) using Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate). |
